The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

Frontiers in Microbiology

Microbiology

Stanley W. Botchway¹  Kathryn Welsby¹  Emily A. White²  Gary J. Sharples²  Ryan Waite²  Carrie A. Ambler³  Joshua G. Hughes⁴  Candace T. Adams⁵  David R. Chisholm⁵  Eva Dias⁵  C. H. Cole Sims⁵  Andrew Whiting⁶ 

[1] Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, United Kingdom;Department of Biosciences, Durham University, Science Site, Durham, United Kingdom;Department of Biosciences, Durham University, Science Site, Durham, United Kingdom;LightOx Limited, Newcastle, United Kingdom;Department of Biosciences, Durham University, Science Site, Durham, United Kingdom;LightOx Limited, Newcastle, United Kingdom;Department of Physics, Durham University, Science Site, Durham, United Kingdom;LightOx Limited, Newcastle, United Kingdom;LightOx Limited, Newcastle, United Kingdom;Department of Chemistry, Durham University, Durham, United Kingdom;

关键词: antimicrobial resistance;    photodynamic therapy;    Gram-positive bacteria;    lipopolysaccharides;    reactive oxygen species;   

DOI  :  10.3389/fmicb.2023.1243818

 received in 2023-06-21, accepted in 2023-09-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

The emergence of antibiotic resistance is a growing threat to human health, and therefore, alternatives to existing compounds are urgently needed. In this context, a novel fluorescent photoactivatable diarylacetylene has been identified and characterised for its antibacterial activity, which preferentially eliminates Gram-positive over Gram-negative bacteria. Experiments confirmed that the Gram-negative lipopolysaccharide-rich outer surface is responsible for tolerance, as strains with reduced outer membrane integrity showed increased susceptibility. Additionally, bacteria deficient in oxidative damage repair pathways also displayed enhanced sensitivity, confirming that reactive oxygen species production is the mechanism of antibacterial activity. This new diarylacetylene shows promise as an antibacterial agent against Gram-positive bacteria that can be activated in situ, potentially for the treatment of skin infections.

【 授权许可】

Unknown   
Copyright © 2023 Waite, Adams, Chisholm, Sims, Hughes, Dias, White, Welsby, Botchway, Whiting, Sharples and Ambler.