Evaluation of a Luminometric Cell Counting System in Context of Antimicrobial Photodynamic Inactivation

Microorganisms

Volker Eulenburg¹  Svitlana Ziganshyna¹  Robert Werdehausen¹  Moritz Lehnig¹  Norman Lippmann²  Sarah Glass³ 

[1] Department of Anaesthesiology and Intensive Care, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany;Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, 04103 Leipzig, Germany;Leibniz Institute of Surface Engineering (IOM), 04318 Leipzig, Germany;

关键词: photodynamic therapy;    photodynamic inactivation;    luminescence measurement;    antimicrobial resistance;    TMPyP;    THPTS;   

DOI  :  10.3390/microorganisms10050950

来源: DOAJ

【 摘 要 】

Antimicrobial resistance belongs to the most demanding medical challenges, and antimicrobial photodynamic inactivation (aPDI) is considered a promising alternative to classical antibiotics. However, the pharmacologic characterization of novel compounds suitable for aPDI is a tedious and time-consuming task that usually requires preparation of bacterial cultures and counting of bacterial colonies. In this study, we established and utilized a luminescence-based microbial cell viability assay to analyze the aPDI effects of two porphyrin-based photosensitizers (TMPyP and THPTS) on several bacterial strains with antimicrobial resistance. We demonstrate that after adaptation of the protocol and initial calibration to every specific bacterial strain and photosensitizer, the luminometric method can be used to reliably quantify aPDI effects in most of the analyzed bacterial strains. The interference of photosensitizers with the luminometric readout and the bioluminescence of some bacterial strains were identified as possible confounders. Using this method, we could confirm the susceptibility of several bacterial strains to photodynamic treatment, including extensively drug-resistant pathogens (XDR). In contrast to the conventional culture-based determination of bacterial density, the luminometric assay allowed for a much more time-effective analysis of various treatment conditions. We recommend this luminometric method for high-throughput tasks requiring measurements of bacterial viability in the context of photodynamic treatment approaches.

【 授权许可】

Unknown