Comparative HPTLC study for simultaneous determination of ivabradine and metoprolol using UV and fluorescence detectors

BMC Chemistry

Research

M. Rizk¹  Maha M. Abou El-Alamin¹  Shereen Mowaka²  Mariam Mohamed³ 

[1] Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt;Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt;Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, 11837, El-Sherouk City, Egypt;Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, 11837, El-Sherouk City, Egypt;

关键词: Ivabradine;    Metoprolol;    HPTLC- densitometry;    Fluorescence detection mode;    UV absorbance detection mode;    Green assessment;   

DOI  :  10.1186/s13065-023-01025-x

 received in 2023-01-09, accepted in 2023-08-29,  发布年份 2023

来源: Springer

【 摘 要 】

New, simple, accurate, sensitive and validated high performance thin layer chromatographic (HPTLC) method coupled with UV absorbance mode and fluorescence (FL) detectors which were used for simultaneous determination of ivabradine (IVA) and metoprolol (MET) in their bulk and pharmaceutical dosage form using TLC silica 60 F254 plates and non-fluorescent TLC silica gel 60 plates. The developing system was chloroform: methanol: formic acid: ammonia (8.5:1.5:0.2:0.1, v/v). Desnitometric analysis in UV absorbance mode was set at λ = 275 nm. While, fluorescence mode was performed with excitation at 260 nm for quantitative simultaneous determination of IVA and MET using optical filter K320. The retention factors values were 0.45 ± 0.05 and 0.89 ± 0.01 of IVA and MET, respectively. UV absorbance mode, linearity was 50.0–600.0 ng/band for IVA and 50.0–900.0 ng/band for MET. For fluorescence mode, the linearity ranges were 18.0–400.0 ng/band and 50.0–550.0 ng/band for IVA and MET; respectively. ICH guidelines were followed in respect to linearity and range, accuracy, precision and selectivity, limit of detection (LOD), limit of quantitation (LOQ) and robustness. The analytical eco-scale, green analytical procedure index (GAPI) and analytical greenness metric tools were used to assess the suggested method. The quantitative proposed method results showed there was no statistically significant difference at 95% confidence when compared to the reported method of high performance liquid chromatography (HPLC).

【 授权许可】

CC BY   
© Springer Nature Switzerland AG 2023

【 图 表 】

Fig. 1

40708_2023_201_Article_IEq9.gif

13690_2023_1170_Article_IEq216.gif

13690_2023_1170_Article_IEq211.gif

Fig. 3

13690_2023_1170_Article_IEq209.gif

Fig. 1

12888_2023_5142_Article_IEq17.gif

12888_2023_5142_Article_IEq3.gif