P3HB from CH4 using methanotrophs: aspects of bioreactor, fermentation process and modelling for cost-effective biopolymer production

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Maximilian Lackner¹  Kianoush Khosravi-Darani²  Parya Safaeian³  Fatemeh Yazdian³  Hamid Rashedi⁴ 

[1] Circe Biotechnologie GmbH, Vienna, Austria;Department of Food Technology Research, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran;

关键词: bioreactor;    poly-3-hydroxybutyrate (P3HB);    polyhydroxyalkanoates (PHA);    vertical tubular loop bioreactor;    horizontal tubular loop bioreactor (HTLB);    forced liquid flow;   

DOI  :  10.3389/fbioe.2023.1137749

 received in 2023-01-04, accepted in 2023-06-01,  发布年份 2023

来源: Frontiers

【 摘 要 】

P3HB (poly-β-hydroxybutyrate), an energy-storage compound of several microorganisms, can be used as bioplastics material. P3HB is completely biodegradable under aerobic and aerobic conditions, also in the marine environment. The intracellular agglomeration of P3HB was examined employing a methanotrophic consortium. Supplanting fossil, non-degradable polymers by P3HB can significantly reduce the environmental impact of plastics. Utilizing inexpensive carbon sources like CH4 (natural gas, biogas) is a fundamental methodology to make P3HB production less costly, and to avoid the use of primary agricultural products such as sugar or starch. Biomass growth in polyhydroxyalkanoates (PHA) in general and in Poly (3-hydroxybutyrate) manufacture in specific could be a foremost point, so here the authors focus on natural gas as a proper carbon source and on the selection of bioreactors to produceP3HB, and in future further PHA, from that substrate. CH4 can also be obtained from biomass, e.g., biogas, syngas methanation or power-to-gas (synthetic natural gas, SNG). Simulation software can be utilized for examination, optimizing and scale-up of the process as shown in this paper. The fermentation systems continuously stirred tank reactor (CSTR), forced-liquid vertical loop bioreactor (VTLB), forced-liquid horizontal tubular loop bioreactor (HTLB), airlift (AL) fermenter and bubble column (BC) fermenter were compared for their methane conversion, kLa value, productivity, advantages and disadvantages. Methane is compared to methanol and other feedstocks. It was discovered that under optimum processing circumstances and using Methylocystis hirsuta, the cells accumulated 51.6% cell dry mass of P3HB in the VTLB setup.

【 授权许可】

Unknown   
Copyright © 2023 Safaeian, Yazdian, Khosravi-Darani, Rashedi and Lackner.