Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

Microorganisms

Joana C. Fradinho¹  Maria A. M. Reis¹  Juliana R. Almeida¹  Gilda Carvalho²  Adrian Oehmen² 

[1] Associate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal;UCIBIO—Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal;

关键词: phototrophic mixed cultures (PMC);    phototrophic purple bacteria (PPB);    polyhydroxyalkanoates (PHA);    feast and famine (FF);    permanent feast (PF);    domestic wastewater;   

DOI  :  10.3390/microorganisms10020351

来源: DOAJ

【 摘 要 】

Phototrophic mixed cultures (PMC) are versatile systems which can be applied for waste streams, valorisation and production of added-value compounds, such as polyhydroxyalkanoates (PHA). This work evaluates the influence of different operational conditions on the bacterial communities reported in PMC systems with PHA production capabilities. Eleven PMCs, fed either with acetate or fermented wastewater, and selected under either feast and famine (FF) or permanent feast (PF) regimes, were evaluated. Overall, results identified Chromatiaceae members as the main phototrophic PHA producers, along with Rhodopseudomonas, Rhodobacter and Rhizobium. The findings show that Chromatiaceae were favoured under operating conditions with high carbon concentrations, and particularly under the PF regime. In FF systems fed with fermented wastewater, the results indicate that increasing the organic loading rate enriches for Rhodopseudomonas, Rhizobium and Hyphomicrobiaceae, which together with Rhodobacter and Chromatiaceae, were likely responsible for PHA storage. In addition, high-sugar feedstock impairs PHA production under PF conditions (fermentative bacteria dominance), which does not occur under FF. This characterization of the communities responsible for PHA accumulation helps to define improved operational strategies for PHA production with PMC.

【 授权许可】

Unknown