Antigen-specific cytokine profiles for pulmonary Mycobacterium avium complex disease stage diagnosis

Frontiers in Immunology

Immunology

Yukiko Ohara¹  Haruka Kobayashi¹  Sohkichi Matsumoto¹  Ikkoh Yasuda²  Yoshiro Yamashita³  Mayumi Terada⁴  Konosuke Morimoto⁵  Shymaa Enany⁶  Yoshiko Tsuchihashi⁷  Norichika Asoh⁷  Toru Ikeda⁸  Masahiro Takaki⁹  Takeshi Tanaka^1,10 

[1] Department of Bacteriology, Niigata University Graduate School of Medicine, Niigata, Niigata, Japan;Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki, Japan;Department of General Internal Medicine and Clinical Infectious Diseases, Fukushima Medical University, Fukushima, Fukushima, Japan;Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki, Japan;Department of Respiratory Medicine, Shunkaikai Inoue Hospital, Nagasaki, Nagasaki, Japan;Department of Internal Medicine, Koseikai Nijigaoka Hospital, Nagasaki, Nagasaki, Japan;Department of Internal Medicine, Koseikai Nijigaoka Hospital, Nagasaki, Nagasaki, Japan;Department of Respiratory Infectious Disease, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki, Japan;Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt;Biomedical Research Department, Armed Force College of Medicine, Cairo, Egypt;Department of Respiratory Medicine, Juzenkai Hospital, Nagasaki, Nagasaki, Japan;Department of Respiratory Medicine, Nagasaki Rosai Hospital, Sasebo, Nagasaki, Japan;Department of Respiratory Medicine, Shunkaikai Inoue Hospital, Nagasaki, Nagasaki, Japan;Infection Control and Education Center, Nagasaki University Hospital, Nagasaki, Nagasaki, Japan;

关键词: Mycobacterium avium;    clinical stage;    Mycobacterium avium;    cell-mediated immunity;    CD4+T cells;    CD19+B cells;    cytokine profile;   

DOI  :  10.3389/fimmu.2023.1222428

 received in 2023-05-14, accepted in 2023-06-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionControlling pulmonary Mycobacterium avium complex (MAC) disease is difficult because there is no way to know the clinical stage accurately. There have been few attempts to use cell-mediated immunity for diagnosing the stage. The objective of this study was to characterize cytokine profiles of CD4+T and CD19+B cells that recognize various Mycobacterium avium-associated antigens in different clinical stages of MAC.MethodsA total of 47 MAC patients at different stages based on clinical information (14 before-treatment, 16 on-treatment, and 17 after-treatment) and 17 healthy controls were recruited. Peripheral blood mononuclear cells were cultured with specific antigens (MAV0968, 1160, 1276, and 4925), and the cytokine profiles (IFN-γ, TNF-α, IL-2, IL-10, IL-13, and IL-17) of CD4+/CD3+ and CD19+ cells were analyzed by flow cytometry.ResultsThe response of Th1 cytokines such as IFN-γ and TNF-α against various antigens was significantly higher in both the on-treatment and after-treatment groups than in the before-treatment group and control (P < 0.01–0.0001 and P < 0.05–0.0001). An analysis of polyfunctional T cells suggested that the presence of IL-2 is closely related to the stage after the start of treatment (P = 0.0309-P < 0.0001) and is involved in memory function. Non-Th1 cytokines, such as IL-10 and IL-17, showed significantly higher responses in the before-treatment group (P < 0.0001 and P < 0.01–0.0001). These responses were not observed with purified protein derivative (PPD). CD19+B cells showed a response similar to that of CD4+T cells.ConclusionThere is a characteristic cytokine profile at each clinical stage of MAC.

【 授权许可】

Unknown   
Copyright © 2023 Yamashita, Yasuda, Tanaka, Ikeda, Terada, Takaki, Tsuchihashi, Asoh, Ohara, Enany, Kobayashi, Matsumoto and Morimoto