Comparison of methods for mycobacteria isolation from swine feces

Brazilian Journal of Microbiology

Eugenia Márcia De Deus Oliveira²  César Alejandro Rosales Rodriguez¹  Vivianne Cambuí Mesquita Rocha¹  Simone Rodriguez Ambrosio¹  Patrícia Miyuki Ohara¹  Marcos Amaku¹  Fernando Ferreira¹  Ricardo Augusto Dias¹  Sylvia Cardoso Leão¹  José Soares Ferreira Neto¹ 

[1] ,Universidade Federal da Bahia Escola de Medicina Veterinária Departamento de Medicina Veterinária PreventivaSalvador BA ,Brasil

关键词: Mycobacterium avium;    isolation;    feces;    swine;    Mycobacterium avium;    isolamento;    fezes;    suínos;   

DOI  :  10.1590/S1517-83822007000400019

来源: SciELO

【 摘 要 】

Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).

【 授权许可】

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