The human lens is capable of trilineage differentiation towards osteo-, chondro-, and adipogenesis—a model for studying cataract pathogenesis

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Gerard Boix-Lemonche¹  Goran Petrovski²  Richard M. Nagymihaly³  Xhevat Lumi⁴ 

[1] Department of Ophthalmology, Center for Eye Research and Innovative Diagnostics, Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway;Department of Ophthalmology, Center for Eye Research and Innovative Diagnostics, Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway;Department of Ophthalmology, Oslo University Hospital, Oslo, Norway;Department of Ophthalmology, University of Split School of Medicine and University Hospital Centre, Split, Croatia;Department of Ophthalmology, Oslo University Hospital, Oslo, Norway;Department of Ophthalmology, Oslo University Hospital, Oslo, Norway;Eye Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia;Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia;

关键词: human lens;    trilineage differentiation;    osteogenesis;    chondrogenesis;    adipogenesis;    cataract;   

DOI  :  10.3389/fbioe.2023.1164795

 received in 2023-02-13, accepted in 2023-05-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

The potential for trilineage differentiation of cells in tissues represents a model for studying disease pathogenesis and regeneration pathways. Human lens trilineage differentiation has not yet been demonstrated, and so has calcification and osteogenic differentiation of human lens epithelial cells in the whole human lens. Such changes can pose a risk for complications during cataract surgery. Human lens capsules (n = 9) from cataract patients undergoing uneventful surgery were trilineage-differentiated toward osteogenesis, chondrogenesis, and adipogenesis. Furthermore, whole human healthy lenses (n = 3) collected from cadaveric eyes were differentiated into bone and characterized by immunohistochemistry. The cells in the human lens capsules were capable of undergoing trilineage differentiation, while the whole human healthy lenses could undergo osteogenesis differentiation, expressing osteocalcin, collagen I, and pigment epithelium-derived factor. We, hereby, show an ex vivo model for cataract formation through different stages of opacification, as well as provide in vivo evidence from patients undergoing calcified lens extraction with bone-like consistency.

【 授权许可】

Unknown   
Copyright © 2023 Boix-Lemonche, Nagymihaly, Lumi and Petrovski.