Inhibitory effects of vaginal Lactobacilli on Candida albicans growth, hyphal formation, biofilm development, and epithelial cell adhesion

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Hiroshige Mikamo¹  Yuka Yamagishi²  Tomonori Takano³  Hiroyuki Kunishima³  Yukitaka Yamasaki³  Takuro Koshikawa⁴  Hiromu Takemura⁴  Motomichi Takahashi⁵  Shuhei Eguchi⁵  Kentaro Oka⁵  Asami Matsumoto⁵  Hayami Kudo⁵ 

[1] Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute, Aichi, Japan;Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute, Aichi, Japan;Department of Clinical Infectious Diseases, Kochi Medical School, Nankoku-shi, Kochi, Japan;Department of Infectious Diseases, St. Marianna University School of Medicine, Kawasaki-shi, Kanagawa, Japan;Department of Microbiology, St. Marianna University School of Medicine, Kawasaki-shi, Japan;Research Department, R&D Division, Miyarisan Pharmaceutical Co., Ltd., Saitama-shi, Saitama, Japan;

关键词: Candida albicans;    Lactobacillus;    biofilm;    probiotics;    cell adhesion;   

DOI  :  10.3389/fcimb.2023.1113401

 received in 2022-12-01, accepted in 2023-04-19,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionAntifungal agents are not always efficient in resolving vulvovaginal candidiasis (VVC), a common genital infection caused by the overgrowth of Candida spp., including Candida albicans, or in preventing recurrent infections. Although lactobacilli (which are dominant microorganisms constituting healthy human vaginal microbiota) are important barriers against VVC, the Lactobacillus metabolite concentration needed to suppress VVC is unknown. Methods We quantitatively evaluated Lactobacillus metabolite concentrations to determine their effect on Candida spp., including 27 vaginal strains of Lactobacillus crispatus, L. jensenii, L. gasseri, Lacticaseibacillus rhamnosus, and Limosilactobacillus vaginalis, with inhibitory abilities against biofilms of C. albicans clinical isolates.ResultsLactobacillus culture supernatants suppressed viable fungi by approximately 24%-92% relative to preformed C. albicans biofilms; however, their suppression differed among strains and not species. A moderate negative correlation was found between Lactobacillus lactate production and biofilm formation, but no correlation was observed between hydrogen peroxide production and biofilm formation. Both lactate and hydrogen peroxide were required to suppress C. albicans planktonic cell growth. Lactobacillus strains that significantly inhibited biofilm formation in culture supernatant also inhibited C. albicans adhesion to epithelial cells in an actual live bacterial adhesion competition test.Discussion Healthy human microflora and their metabolites may play important roles in the development of new antifungal agent against C. albicans-induced VVC.

【 授权许可】

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Copyright © 2023 Takano, Kudo, Eguchi, Matsumoto, Oka, Yamasaki, Takahashi, Koshikawa, Takemura, Yamagishi, Mikamo and Kunishima