The Discovery of a Potential Antimicrobial Agent: the Novel Compound Natural Medicinal Plant Fermentation Extracts against Candida albicans

5th Annual International Conference on Material Science and Environmental Engineering

材料科学;生态环境科学

Song, Mingzhu^1,2 ; Wang, Xirui^3 ; Mao, Canquan^4 ; Yao, Wei^1

School of Medicine, Chengdu University, Chengdu

610106, China^1

National Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu

610041, China^2

College of Tourism, Economics and Management, Chengdu University, Chengdu

610106, China^3

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China^4

关键词: ALkaline phosphatase;    Anti-fungal properties;    Anti-microbial effects;    Antimicrobial drug;    Candida albicans;    Membrane integrity;    Minimal inhibitory concentration;    Ultraviolet absorption;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/301/1/012026/pdf
DOI  :  10.1088/1757-899X/301/1/012026

来源: IOP

【 摘 要 】

Natural medicinal plants and their extracts are important sources of antimicrobial drug development. In this study, we reported an ancient formula of Chinese folk medicine, the compound natural medicinal plant fermentation extracts (CNMPFE) for its antimicrobial effects. The effects and mechanisms of CNMPFE on C. albicans were studied by cell damage experiments including antimicrobial kinetics, fungal growth curve, alkaline phosphatase (AKP) activity, ultraviolet absorption, electric conductivity and the evaluation of cellular ultra microstructure. The results showed that the minimal inhibitory concentration and minimum fungicidal concentration of CNMPFE against C. albicans were 75% (vol/vol) and 80% (vol/vol) respectively. The inhibition of CNMPFE for C. albicans was dose and time dependent, based on increasing of the AKP activities and the ultraviolet absorptions and the electric conductivities of the fungal solutions, it may exert its antifungal properties by disrupting the structure of cell wall and the cell membrane integrity and their permeability, subsequently resulting in cell death. Taken together, these findings suggest that CNMPFE may be a promising drug candidate for the treatment of fungal infections skin diseases.