Cold-tolerant phosphate-solubilizing Pseudomonas strains promote wheat growth and yield by improving soil phosphorous (P) nutrition status

Frontiers in Microbiology

Microbiology

Sumit Chaturvedi¹  V. K. Sah¹  Arun Kumar²  Najat A. Bukhari³  Kahkashan Perveen³  Hemant Dasila⁴  Lakshmi Tewari⁵  Manvika Sahgal⁵  Gohar Taj⁶  Vandana Jaggi⁷  Tan Ching Siang⁸ 

[1] Department of Agronomy, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, India;Department of Agronomy, Dr. Khem Singh Gill, Akal College of Agriculture, Eternal University, Rajgarh, Himachal Pradesh, India;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Microbiology, Akal College of Basic Sciences, Eternal University, Rajgarh, Himachal Pradesh, India;Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, India;Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, India;Department of Molecular Biology and Biotechnology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, India;Department of Pediatrics and Human Development, Michigan State University, Grand Rapids, MI, United States;School of Pharmacy, KPJ Healthcare University College, Nilai, Malaysia;

关键词: PSB;    fertilizer;    psychrotroph;    principal component analysis;    response surface methodology;   

DOI  :  10.3389/fmicb.2023.1135693

 received in 2023-01-01, accepted in 2023-02-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

It is well-known that phosphate-solubilizing bacteria (PSB) promote crop growth and yield. The information regarding characterization of PSB isolated from agroforestry systems and their impact on wheat crops under field conditions is rarely known. In the present study, we aim to develop psychrotroph-based P biofertilizers, and for that, four PSB strains (Pseudomonas sp. L3, Pseudomonas sp. P2, Streptomyces sp. T3, and Streptococcus sp. T4) previously isolated from three different agroforestry zones and already screened for wheat growth under pot trial conditions were evaluated on wheat crop under field conditions. Two field experiments were employed; set 1 includes PSB + recommended dose of fertilizers (RDF) and set 2 includes PSB – RDF. In both field experiments, the response of the PSB-treated wheat crop was significantly higher compared to the uninoculated control. In field set 1, an increase of 22% in grain yield (GY), 16% in biological yield (BY), and 10% in grain per spike (GPS) was observed in consortia (CNS, L3 + P2) treatment, followed by L3 and P2 treatments. Inoculation of PSB mitigates soil P deficiency as it positively influences soil alkaline phosphatase (AP) and soil acid phosphatase (AcP) activity which positively correlated with grain NPK %. The highest grain NPK % was reported in CNS-treated wheat with RDF (N–0.26%, P–0.18%, and K-1.66%) and without RDF (N-0.27, P-0.26, and K-1.46%), respectively. All parameters, including soil enzyme activities, plant agronomic data, and yield data were analyzed by principal component analysis (PCA), resulting in the selection of two PSB strains. The conditions for optimal P solubilization, in L3 (temperature-18.46, pH–5.2, and glucose concentration–0.8%) and P2 (temperature-17°C, pH–5.0, and glucose concentration–0.89%), were obtained through response surface methodology (RSM) modeling. The P solubilizing potential of selected strains at <20°C makes them a suitable candidate for the development of psychrotroph-based P biofertilizers. Low-temperature P solubilization of the PSB strains from agroforestry systems makes them potential biofertilizers for winter crops.

【 授权许可】

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Copyright © 2023 Dasila, Sah, Jaggi, Kumar, Tewari, Taj, Chaturvedi, Perveen, Bukhari, Siang and Sahgal.