FEBS Letters | |
Ultraviolet resonance Raman evidence for the absence of tyrosinate in octopus rhodopsin and the participation of Trp residues in the transition to acid metarhodopsin | |
Nakagawa, Masashi1  Hashimoto, Shinji2  Takeuchi, Hideo2  Tsuda, Motoyuki1  | |
[1] Department of Life Science, Himeji Institute of Technology, Hyogo 678-12, Japan;Pharmaceutical Institute, Tohoku University, Aobayama, Sendai 980-77, Japan | |
关键词: Octopus rhodopsin; Ultraviolet resonance Raman spectroscopy; Protonated Schiff base; Tyrosine; Tryptophan; MES; 2-morpholinoethanesulfonic acid; PSB; protonated Schiff base; Tyr−; tyrosinate; UVRR; ultraviolet resonance Raman; λ max; absorption maximum wavelength; | |
DOI : 10.1016/S0014-5793(96)01250-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The ultraviolet (244 nm) resonance Raman spectra of octopus rhodopsin and its photoproduct, acid metarhodopsin, do not give any evidence for a tyrosinate. This finding excludes the possibility that Tyr-112 serves as the counter anion to the protonated Schiff base as does Glu-113 in bovine rhodopsin. Upon photoconversion from rhodopsin to acid metarhodopsin, Trp and Tyr Raman bands decrease in intensity and concomitantly a Trp band shifts in frequency. The changes of Trp Raman bands are ascribed to changes in hydrophobic interactions and conformation, suggesting a possible role of Trp in the photoconversion process of octopus rhodopsin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303612ZK.pdf | 458KB | download |