Ultraviolet resonance Raman evidence for the absence of tyrosinate in octopus rhodopsin and the participation of Trp residues in the transition to acid metarhodopsin

FEBS Letters

Nakagawa, Masashi¹  Hashimoto, Shinji²  Takeuchi, Hideo²  Tsuda, Motoyuki¹ 

[1] Department of Life Science, Himeji Institute of Technology, Hyogo 678-12, Japan;Pharmaceutical Institute, Tohoku University, Aobayama, Sendai 980-77, Japan

关键词: Octopus rhodopsin;    Ultraviolet resonance Raman spectroscopy;    Protonated Schiff base;    Tyrosine;    Tryptophan;    MES;    2-morpholinoethanesulfonic acid;    PSB;    protonated Schiff base;    Tyr−;    tyrosinate;    UVRR;    ultraviolet resonance Raman;    λ max;    absorption maximum wavelength;   

DOI  :  10.1016/S0014-5793(96)01250-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ultraviolet (244 nm) resonance Raman spectra of octopus rhodopsin and its photoproduct, acid metarhodopsin, do not give any evidence for a tyrosinate. This finding excludes the possibility that Tyr-112 serves as the counter anion to the protonated Schiff base as does Glu-113 in bovine rhodopsin. Upon photoconversion from rhodopsin to acid metarhodopsin, Trp and Tyr Raman bands decrease in intensity and concomitantly a Trp band shifts in frequency. The changes of Trp Raman bands are ascribed to changes in hydrophobic interactions and conformation, suggesting a possible role of Trp in the photoconversion process of octopus rhodopsin.

【 授权许可】

Unknown