Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey

Marine Drugs

Lidiane S. Araújo²  Edna Kagohara²  Thaís P. Garcia²  Vivian H. Pellizari¹ 

[1] Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico 191, SP 05508-120, São Paulo, Brazil; E-Mail:;Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900, São Paulo, Brazil; E-Mails:

关键词: oxidoreductases;    chiral alcohols;    psychrophile;    psychrotroph;    oxidation;    deracemisation;   

DOI  :  10.3390/md9050889

来源: mdpi

【 摘 要 】

Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.