Cassava begomovirus species diversity changes during plant vegetative cycles

Frontiers in Microbiology

Microbiology

Ignazio Carbone¹  Siobain Duffy²  J. Steen Hoyer²  Alana Jacobson³  George Kennedy⁴  Paul Labadie⁴  Jenniffer Mwangi⁵  Brenda Muga⁵  Elijah Ateka⁵  Vanessa Ly⁶  José Trinidad Ascencio-Ibáñez⁶  Yamilex Rosado⁶  Louis Jackai⁷  Anna E. Dye⁸  Linda Hanley-Bowdoin⁸  David Deppong⁸  Benard Mware⁹  Mary Wambugu⁹  William Sharpee^1,10 

[1] Center for Integrated Fungal Research, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States;Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ, United States;Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, United States;Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States;Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya;Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, United States;Department of Natural Resources and Environmental Design, North Carolina Agricultural and Technical State University, Greensboro, NC, United States;Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States;International Livestock Research Institute (ILRI), Nairobi, Kenya;International Livestock Research Institute (ILRI), Nairobi, Kenya;Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States;

关键词: plant virus;    Manihot esculenta;    vegetative (asexual) propagation;    vector transmission;    Bemisia tabaci;   

DOI  :  10.3389/fmicb.2023.1163566

 received in 2023-02-10, accepted in 2023-04-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

Cassava is a root crop important for global food security and the third biggest source of calories on the African continent. Cassava production is threatened by Cassava mosaic disease (CMD), which is caused by a complex of single-stranded DNA viruses (family: Geminiviridae, genus: Begomovirus) that are transmitted by the sweet potato whitefly (Bemisia tabaci). Understanding the dynamics of different cassava mosaic begomovirus (CMB) species through time is important for contextualizing disease trends. Cassava plants with CMD symptoms were sampled in Lake Victoria and coastal regions of Kenya before transfer to a greenhouse setting and regular propagation. The field-collected and greenhouse samples were sequenced using Illumina short-read sequencing and analyzed on the Galaxy platform. In the field-collected samples, African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Kenya virus (EACMKV), and East African cassava mosaic virus-Uganda variant (EACMV-Ug) were detected in samples from the Lake Victoria region, while EACMV and East African mosaic Zanzibar virus (EACMZV) were found in the coastal region. Many of the field-collected samples had mixed infections of EACMV and another begomovirus. After 3 years of regrowth in the greenhouse, only EACMV-like viruses were detected in all samples. The results suggest that in these samples, EACMV becomes the dominant virus through vegetative propagation in a greenhouse. This differed from whitefly transmission results. Cassava plants were inoculated with ACMV and another EACMV-like virus, East African cassava mosaic Cameroon virus (EACMCV). Only ACMV was transmitted by whiteflies from these plants to recipient plants, as indicated by sequencing reads and copy number data. These results suggest that whitefly transmission and vegetative transmission lead to different outcomes for ACMV and EACMV-like viruses.

【 授权许可】

Unknown   
Copyright © 2023 Dye, Muga, Mwangi, Hoyer, Ly, Rosado, Sharpee, Mware, Wambugu, Labadie, Deppong, Jackai, Jacobson, Kennedy, Ateka, Duffy, Hanley-Bowdoin, Carbone and Ascencio-Ibáñez.