Severe Stunting Symptoms upon Nepovirus Infection Are Reminiscent of a Chronic Hypersensitive-like Response in a Perennial Woody Fruit Crop

Viruses

Corinne Schmitt-Keichinger¹  Isabelle R. Martin¹  Philippe Hugueney¹  Amandine Velt¹  Véronique Komar¹  Olivier Lemaire¹  Shahinez Garcia¹  Raymonde Baltenweck¹  Jean-Michel Hily¹  Emmanuelle Vigne¹  Camille Rustenholz¹ 

[1] Santé de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, France;

关键词: contrasting phenotypes;    grapevine;    hypersensitive response;    metabolome;    pathogenicity;    plant virus;   

DOI  :  10.3390/v13112138

来源: DOAJ

【 摘 要 】

Virus infection of plants can result in various degrees of detrimental impacts and disparate symptom types and severities. Although great strides have been made in our understanding of the virus–host interactions in herbaceous model plants, the mechanisms underlying symptom development are poorly understood in perennial fruit crops. Grapevine fanleaf virus (GFLV) causes variable symptoms in most vineyards worldwide. To better understand GFLV-grapevine interactions in relation to symptom development, field and greenhouse trials were conducted with a grapevine genotype that exhibits distinct symptoms in response to a severe and a mild strain of GFLV. After validation of the infection status of the experimental vines by high-throughput sequencing, the transcriptomic and metabolomic profiles in plants infected with the two viral strains were tested and compared by RNA-Seq and LC-MS, respectively, in the differentiating grapevine genotype. In vines infected with the severe GFLV strain, 1023 genes, among which some are implicated in the regulation of the hypersensitive-type response, were specifically deregulated, and a higher accumulation of resveratrol and phytohormones was observed. Interestingly, some experimental vines restricted the virus to the rootstock and remained symptomless. Our results suggest that GFLV induces a strain- and cultivar-specific defense reaction similar to a hypersensitive reaction. This type of defense leads to a severe stunting phenotype in some grapevines, whereas others are resistant. This work is the first evidence of a hypersensitive-like reaction in grapevine during virus infection.

【 授权许可】

Unknown