Frontiers in Plant Science | |
3D cellular morphometrics of ovule primordium development in Zea mays reveal differential division and growth dynamics specifying megaspore mother cell singleness | |
Plant Science | |
Jean-Luc Verdeil1  Marc Lartaud1  Inès Ouedraogo2  Oliver Leblanc2  Daphné Autran2  Luciana Delgado3  Geneviève Conéjéro4  Gabriella Mosca5  Célia Baroux5  | |
[1] AGAP, University of Montpellier, CIRAD, INRAE, Institut SupAgro, Montpellier, France;DIADE, University of Montpellier, IRD, CIRAD, Montpellier, France;IICAR, CONICET, University of Rosario, Rosario, Argentina;IPSIM, University of Montpellier, CNRS, INRAE, Institut SupAgro, Montpellier, France;Institute of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland; | |
关键词: ovule primordium; 3D morphometrics; developmental atlas; morphogenesis; Zea mays; female germ cell; MMC specification; | |
DOI : 10.3389/fpls.2023.1174171 | |
received in 2023-02-26, accepted in 2023-04-07, 发布年份 2023 | |
来源: Frontiers | |
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【 摘 要 】
IntroductionDifferentiation of spore mother cells marks the somatic-to-reproductive transition in higher plants. Spore mother cells are critical for fitness because they differentiate into gametes, leading to fertilization and seed formation. The female spore mother cell is called the megaspore mother cell (MMC) and is specified in the ovule primordium. The number of MMCs varies by species and genetic background, but in most cases, only a single mature MMC enters meiosis to form the embryo sac. Multiple candidate MMC precursor cells have been identified in both rice and Arabidopsis, so variability in MMC number is likely due to conserved early morphogenetic events. In Arabidopsis, the restriction of a single MMC per ovule, or MMC singleness, is determined by ovule geometry. To look for potential conservation of MMC ontogeny and specification mechanisms, we undertook a morphogenetic description of ovule primordium growth at cellular resolution in the model crop maize.MethodsWe generated a collection of 48 three-dimensional (3D) ovule primordium images for five developmental stages, annotated for 11 cell types. Quantitative analysis of ovule and cell morphological descriptors allowed the reconstruction of a plausible developmental trajectory of the MMC and its neighbors.ResultsThe MMC is specified within a niche of enlarged, homogenous L2 cells, forming a pool of candidate archesporial (MMC progenitor) cells. A prevalent periclinal division of the uppermost central archesporial cell formed the apical MMC and the underlying cell, a presumptive stack cell. The MMC stopped dividing and expanded, acquiring an anisotropic, trapezoidal shape. By contrast, periclinal divisions continued in L2 neighbor cells, resulting in a single central MMC.DiscussionWe propose a model where anisotropic ovule growth in maize drives L2 divisions and MMC elongation, coupling ovule geometry with MMC fate.
【 授权许可】
Unknown
Copyright © 2023 Ouedraogo, Lartaud, Baroux, Mosca, Delgado, Leblanc, Verdeil, Conéjéro and Autran
【 预 览 】
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