Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium

eLife

Caroline Michaud¹  Daniel Grimanelli¹  Elvira Hernandez-Lagana¹  Daphné Autran²  Gabriella Mosca³  Anja Frey³  Alejandro Giraldo-Fonseca³  Ueli Grossniklaus³  Nuno Pires³  Célia Baroux³  Ethel Mendocilla-Sato³  Olivier Hamant⁴  Christophe Godin⁴  Arezki Boudaoud⁴ 

[1] DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France;DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France;Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, France;Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland;Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, France;

关键词: ovule primordium;    germline;    cell fate;    plasticity;    growth;    tissue geometry;    A. thaliana;   

DOI  :  10.7554/eLife.66031

来源: eLife Sciences Publications, Ltd

【 摘 要 】

In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium forms. Tissue growth models uncovered plausible morphogenetic principles involving a spatially confined growth signal, differential mechanical properties, and cell growth anisotropy. Our analysis revealed that SMC characteristics first arise in more than one cell but SMC fate becomes progressively restricted to a single cell during organ growth. Altered primordium geometry coincided with a delay in the fate restriction process in katanin mutants. Altogether, our study suggests that tissue geometry channels reproductive cell fate in the Arabidopsis ovule primordium.

【 授权许可】

CC BY