Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium

eLife

Célia Baroux¹  Elvira Hernandez-Lagana²  Caroline Michaud²  Daphné Autran²  Daniel Grimanelli²  Ethel Mendocilla-Sato³  Ueli Grossniklaus³  Anja Frey³  Gabriella Mosca³  Alejandro Giraldo-Fonseca³  Nuno Pires³  Olivier Hamant⁴  Christophe Godin⁴  Arezki Boudaoud⁴ 

[1] Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, France;DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France;Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland;Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, France;

关键词: ovule primordium;    germline;    cell fate;    plasticity;    growth;    tissue geometry;   

DOI  :  10.7554/eLife.66031

来源: DOAJ

【 摘 要 】

In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium forms. Tissue growth models uncovered plausible morphogenetic principles involving a spatially confined growth signal, differential mechanical properties, and cell growth anisotropy. Our analysis revealed that SMC characteristics first arise in more than one cell but SMC fate becomes progressively restricted to a single cell during organ growth. Altered primordium geometry coincided with a delay in the fate restriction process in katanin mutants. Altogether, our study suggests that tissue geometry channels reproductive cell fate in the Arabidopsis ovule primordium.

【 授权许可】

Unknown