Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control

Frontiers in Pharmacology

Pharmacology

Chunyun Yuan¹  Jian Yi²  Bowei Chen²  Yingfei Liu²  Yaqian Xu²  Fengming Tian²  Yin Ouyang²  Baiyan Liu³ 

[1]Hunan Hospital of Integrated Traditional Chinese and Western Medicine, Changsha, China

[2]Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha, China

[3]The First Hospital of Hunan University of Chinese Medicine, Changsha, China

[4]MOE Key Laboratory of Research and Translation on Prevention and Treatment of Major Diseases in Internal Medicine of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China

[5]The First Hospital of Hunan University of Chinese Medicine, Changsha, China

[6]MOE Key Laboratory of Research and Translation on Prevention and Treatment of Major Diseases in Internal Medicine of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China

[7]Hunan Academy of Chinese Medicine, Changsha, China

关键词: Buyang Huanwu Decoction;    caveolin-1;    cerebral ischemia;    mitochondrial quality control;    mitochondrial dynamics;    mitophagy;    mitochondrial biogenesis;   

DOI  :  10.3389/fphar.2023.1137609

 received in 2023-01-04, accepted in 2023-04-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Mitochondrial quality control (MQC) is an important mechanism of neural repair after cerebral ischemia (CI). Recent studies have shown that caveolin-1 (Cav-1) is an important signaling molecule in the process of CI injury, but its mechanism of regulating MQC after CI is still unclear. Buyang Huanwu Decoction (BHD) is a classic traditional Chinese medicine formula that is often used to treat CI. Unfortunately, its mechanism of action is still obscure.Methods: In this study, we tested the hypothesis that BHD can regulate MQC through Cav-1 and exert an anti-cerebral ischemia injury effect. We used Cav-1 knockout mice and their homologous wild-type mice, replicated middle cerebral artery occlusion (MCAO) model and BHD intervention. Neurobehavioral scores and pathological detection were used to evaluate neurological function and neuron damage, transmission electron microscopy and enzymology detection of mitochondrial damage. Finally, western blot and RT-qPCR expression of MQC-related molecules were tested.Results: After CI, mice showed neurologic impairment, neuronal damage, and significant destruction of mitochondrial morphology and function, and MQC was imbalanced. Cav-1 deletion aggravated the damage to neurological function, neurons, mitochondrial morphology and mitochondrial function after CI, aggravated the imbalance of mitochondrial dynamics, and inhibited mitophagy and biosynthesis. BHD can maintain MQC homeostasis after CI through Cav-1 and improve CI injury.Discussion: Cav-1 can affect CI injury by regulating MQC, and this mechanism may be another target of BHD for anti-cerebral ischemia injury.

【 授权许可】

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Copyright © 2023 Xu, Chen, Yi, Tian, Liu, Ouyang, Yuan and Liu.