Neuroprotective effect of Buyang Huanwu Decoction on spinal ischemia-reperfusion injury in rats is linked with inhibition of cyclin-dependent kinase 5

BMC Complementary and Alternative Medicine

Dian-Ming Jiang¹  Lei Wang¹ 

[1]Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, #1 Youyi Rd, Chongqing 400016, China

关键词: Roscovitine;    Inhibitor;    Cdk5;    Spinal ischemia-reperfusion;    Buyang Huanwu Decoction;   

Others  :  1220806
DOI  :  10.1186/1472-6882-13-309

 received in 2013-05-10, accepted in 2013-10-29,  发布年份 2013

【 摘 要 】

Background

Buyang Huanwu Decoction (BYHWD), a traditional Chinese medicine formula, has been shown to exert a variety of pharmacological effects including neuroprotective properties. However, the mechanism of neuroprotection is not fully understood. This study was designed to explore the mechanism of BYHWD in the treatment of spinal ischemia-reperfusion injury in rats.

Methods

Twenty-eight male Sprague–Dawley rats, weighting 250–280 g, were used, and were randomly divided into four groups with 7 animals in each: sham operation group (Control), spinal ischemia with saline (SI + Saline), spinal ischemia with BYHWD (SI + BYHWD), and spinal ischemia with roscovitine (SI + R). After 60 minutes of spinal ischemia followed by 72 hours of reperfusion, motor function of hind limbs, spinal ischemic infarction volume, the number of apoptotic cells, and cyclin-dependent kinase 5 (Cdk5) were examined.

Result

Ischemia-reperfusion resulted in injury of the spines, while BYHWD significantly improved spinal function. The spinal infarction volume, number of apoptotic cells, and Cdk5 were decreased by administration of BYHWD. The similar improvements were seen with the pre-treatment of roscovitine.

Conclusions

BYHWD prevented the ischemia-reperfusion-induced spinal injury in rats. The protective function of BYHWD was, in part, linked with inhibition of Cdk5.

【 授权许可】

   
2013 Wang and Jiang; licensee BioMed Central Ltd.

【 图 表 】

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