Enhanced nitrogen removal via Yarrowia lipolytica-mediated nitrogen and related metabolism of Chlorella pyrenoidosa from wastewater

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Huifan Liu¹  Qin Wang¹  Danni Lin¹  Lukai Ma¹  Hui Liu²  Yuming Zhong²  Sufen Li³ 

[1] College of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China;College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China;Institute of Water Environment Engineering, Xinhua College of Sun Yat-Sen University, Guangzhou, Guangdong, China;

关键词: Chlorella Pyrenoidosa;    co-cluture;    Yarrowia Lipolytica;    transcriptomics;    nitrogen metabolism pathway;   

DOI  :  10.3389/fbioe.2023.1159297

 received in 2023-02-05, accepted in 2023-05-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

We investigated the optimum co-culture ratio with the highest biological nitrogen removal rate, revealing that chemical oxygen demand, total nitrogen (TN), and ammoniacal nitrogen (NH3-N) removal was increased in the Chlorella pyrenoidosa and Yarrowia lipolytica co-culture system at a 3:1 ratio. Compared with the control, TN and NH3-N content in the co-incubated system was decreased within 2–6 days. We investigated mRNA/microRNA (miRNA) expression in the C. pyrenoidosa and Y. lipolytica co-culture after 3 and 5 days, identifying 9885 and 3976 differentially expressed genes (DEGs), respectively. Sixty-five DEGs were associated with Y. lipolytica nitrogen, amino acid, photosynthetic, and carbon metabolism after 3 days. Eleven differentially expressed miRNAs were discovered after 3 days, of which two were differentially expressed and their target mRNA expressions negatively correlated with each other. One of these miRNAs regulates gene expression of cysteine dioxygenase, hypothetical protein, and histone-lysine N-methyltransferase SETD1, thereby reducing amino acid metabolic capacity; the other miRNA may promote upregulation of genes encoding the ATP-binding cassette, subfamily C (CFTR/MRP), member 10 (ABCC10), thereby promoting nitrogen and carbon transport in C. pyrenoidosa. These miRNAs may further contribute to the activation of target mRNAs. miRNA/mRNA expression profiles confirmed the synergistic effects of a co-culture system on pollutant disposal.

【 授权许可】

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Copyright © 2023 Zhong, Lin, Li, Wang, Liu, Ma and Liu.