Investigating the L-asparaginase Production in the Yeast Yarrowia ‎Lipolytica

Biological Journal of Microorganism

Farshad Darvishi¹  Fereshteh Shamsi¹ 

[1] Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran.;

关键词: Yarrowia Lipolytica;    Asparaginase;    Anticancer Drug;    Production. ‎;   

DOI  :  10.22108/bjm.2018.111339.1133

来源: DOAJ

【 摘 要 】

Introduction: L-Asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also used in the production of acrylamide-free foods. Considering the important applications of this enzyme, the aim of this study was to introduce a new microbial source for the production of eukaryotic L-asparaginase. Materials and Methods: The quality and quantity production of L-asparaginase was investigated in four strains of yeast Yarrowia lipolytica. The quality assessment of L-asparaginase production was done on asparagine minimal agar. Quantitative assay of the enzyme was done in Czapex Dox’s medium by spectrophotometric method using Nessler’s reagent. Results: All of four strains show that they are able to produce asparaginase making a purple halo around the colony. Y. lipolytica DSM3286, DSM70562, DSM3218 and CBS6303 were produced 17/14, 11, 6/98 and 5/61 U/mL of asparaginase in Czapex Dox’s medium after 24 h, respectively. Discussion and Conclusion: Y. lipolytica DSM3286 was selected as the best asparaginase producer strain with the largest halo and the highest asparaginase production. The Y. lipolytica could be used as a potential source of L-asparaginase production.

【 授权许可】

Unknown