Final Technical Report for Award # ER64999

Metcalf, William W.¹ 

[1] University of Illinois

关键词: methane;    methanogenic archaea;    systems biology;    genomics;    transcriptomics;    metabolic modelling;   

DOI  :  10.2172/1159247
RP-ID  :  DOE-UIUC-SC5348
PID  :  OSTI ID: 1159247

美国|英语

来源: SciTech Connect

【 摘 要 】

This report provides a summary of activities for Award # ER64999, a Genomes to Life Project funded by the Office of Science, Basic Energy Research. The project was entitled "Methanogenic archaea and the global carbon cycle: a systems biology approach to the study of Methanosarcina species". The long-term goal of this multi-investigator project was the creation of integrated, multiscale models that accurately and quantitatively predict the role of Methanosarcina species in the global carbon cycle under dynamic environmental conditions. To achieve these goals we pursed four specific aims: (1) genome sequencing of numerous members of the Order Methanosarcinales, (2) identification of genomic sources of phenotypic variation through in silico comparative genomics, (3) elucidation of the transcriptional networks of two Methanosarcina species, and (4) development of comprehensive metabolic network models for characterized strains to address the question of how metabolic models scale with genetic distance.