In vitro activities of antimicrobial combinations against planktonic and biofilm forms of Stenotrophomonas maltophilia

Frontiers in Microbiology

Microbiology

Chien-Ming Chao¹  Chi-Chung Chen²  Hung-Jen Tang³  Chih-Cheng Lai⁴  Chia-Hung Tsai⁵  Hung-Jui Chen⁵  Hsin-Yu Lai⁵  Bo-An Su⁶ 

[1] Department of Intensive Care Medicine, Chi Mei Medical Center, Liouying, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan;Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;Department of Medicine, Chi Mei Medical Center, Tainan, Taiwan;Division of Hospital Medicine, Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan;School of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan;Division of Infectious Diseases, Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan;Division of Infectious Diseases, Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan;Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan, Taiwan;

关键词: Stenotrophomonas maltophilia;    levofloxacin;    fosfomycin;    ceftazidime-avibactam;    tigecycline;   

DOI  :  10.3389/fmicb.2023.1186669

 received in 2023-03-15, accepted in 2023-05-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectivesTo investigate the in vitro activity of antibiotic combinations against Stenotrophomonas maltophilia isolates and their associated biofilms.MethodsThirty-two S. maltophilia clinical isolates with at least twenty-five different pulsotypes were tested. The antibacterial activity of various antibiotic combinations against seven randomly selected planktonic and biofilm-embedded S. maltophilia strains with strong biofilm formation was assessed using broth methods. Extraction of bacterial genomic DNA and PCR detection of antibiotic resistance and biofilm-related genes were also performed.ResultsThe susceptibility rates of levofloxacin (LVX), fosfomycin (FOS), tigecycline (TGC) and sulfamethoxazole-trimethoprim (SXT) against 32 S. maltophilia isolates were 56.3, 71.9, 71.9 and 90.6%, respectively. Twenty-eight isolates were detected with strong biofilm formation. Antibiotic combinations, including aztreonam-clavulanic (ATM-CLA) with LVX, ceftazidime-avibactam (CZA) with LVX and SXT with TGC, exhibited potent inhibitory activity against these isolates with strong biofilm formation. The antibiotic resistance phenotype might not be fully caused by the common antibiotic-resistance or biofilm-formation gene.ConclusionS. maltophilia remained resistant to most antibiotics, including LVX and β-lactam/β-lactamases; however, TGC, FOS and SXT still exhibited potent activity. Although all tested S. maltophilia isolates exhibited moderate-to-strong biofilm formation, combination therapies, especially ATM-CLA with LVX, CZA with LVX and SXT with TGC, exhibited a higher inhibitory activity for these isolates.

【 授权许可】

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Copyright © 2023 Su, Chen, Chen, Lai, Tsai, Lai, Tang and Chao.