Spatial genomics reveals a high number and specific location of B cells in the pancreatic ductal adenocarcinoma microenvironment of long-term survivors

Frontiers in Immunology

Immunology

Marta Mendiola¹  Jaime Feliu²  Andrew P. Stubbs³  Dana A. M. Mustafa⁴  Lawlaw Saida⁴  Yunlei Li⁵  Willem de Koning⁵  Elena Palacios⁶  Hosein M. Aziz⁷  Casper H. J. van Eijck⁸  Kostandinos Sideras⁹ 

[1] Centro de Investigación Biomédica en red de Cáncer, CIBERONC, Instituto de Salud Carlos III, Madrid, Spain;Molecular Pathology and therapeutic Targets Group, La Paz University Hospital, IdiPAZ, Madrid, Spain;Department of Medical Oncology, La Paz University Hospital, IdiPAZ, Madrid, Spain;Cátedra UAM-ANGEM, Madrid, Spain;Centro de Investigación Biomédica en red de Cáncer, CIBERONC, Instituto de Salud Carlos III, Madrid, Spain;Department of Pathology & Clinical Bioinformatics, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Pathology & Clinical Bioinformatics, The Tumor Immuno-Pathology Laboratory, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Pathology & Clinical Bioinformatics, The Tumor Immuno-Pathology Laboratory, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Pathology & Clinical Bioinformatics, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Pathology, La Paz University Hospital, IdiPAZ, Madrid, Spain;Department of Surgery, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Surgery, Erasmus University Medical Center, Rotterdam, Netherlands;Department of Pathology & Clinical Bioinformatics, The Tumor Immuno-Pathology Laboratory, Erasmus University Medical Center, Rotterdam, Netherlands;Divisions of Medical Oncology and Hematology, Mayo Clinic, Rochester, MN, United States;

关键词: pancreatic ductal adenocarcinoma;    long-term survival;    B cells;    tumor immune microenvironment;    gene expression;    spatial genomics;   

DOI  :  10.3389/fimmu.2022.995715

 received in 2022-07-16, accepted in 2022-11-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Background and aimOnly 10% of pancreatic ductal adenocarcinoma (PDAC) patients survive longer than five years. Factors underlining long-term survivorship in PDAC are not well understood. Therefore, we aimed to identify the key players in the tumor immune microenvironment (TIME) associated with long-term survivorship in PDAC patients.MethodsThe immune-related gene expression profiles of resected PDAC tumors of patients who survived and remained recurrence-free of disease for ≥36 months (long-term survivors, n=10) were compared to patients who had survived ≤6 months (short-term survivors, n=10) due to tumor recurrence. Validation was performed by the spatial protein expression profile of immune cells using the GeoMx™ Digital Spatial Profiler. An independent cohort of samples consisting of 12 long-term survivors and 10 short-term survivors, was used for additional validation. The independent validation was performed by combining qualitative immunohistochemistry and quantitative protein expression profiling.ResultsB cells were found to be significantly increased in the TIME of long-term survivors by gene expression profiling (p=0.018). The high tumor infiltration of B cells was confirmed by spatial protein profiling in the discovery and the validation cohorts (p=0.002 and p=0.01, respectively). The higher number of infiltrated B cells was found mainly in the stromal compartments of PDAC samples and was exclusively found within tumor cells in long-term survivors.ConclusionThis is the first comprehensive study that connects the immune landscape of gene expression profiles and protein spatial infiltration with the survivorship of PDAC patients. We found a higher number and a specific location of B cells in TIME of long-term survivors which emphasizes the importance of B cells and B cell-based therapy for future personalized immunotherapy in PDAC patients.

【 授权许可】

Unknown   
Copyright © 2023 Aziz, Saida, de Koning, Stubbs, Li, Sideras, Palacios, Feliu, Mendiola, van Eijck and Mustafa