Genetic characterization of MDR genomic elements carrying two aac(6′)-aph(2″) genes in feline-derived clinical Enterococcus faecalis isolate

Frontiers in Microbiology

Microbiology

Xuan-yu Li¹  Yu Qi¹  Peng-hui Li¹  Xue-Song Li¹  Ya-zhuo Li¹  Dao-mi Zhu¹  Ling-Cong Kong¹  Jun-ze Xue¹  Inam Muhammad²  Hong-Xia Ma³  Ying Ma⁴ 

[1] Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China;The Key Laboratory of New Veterinary Drug Research and Development of Jilin Province, Jilin Agricultural University, Changchun, China;Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China;The Key Laboratory of New Veterinary Drug Research and Development of Jilin Province, Jilin Agricultural University, Changchun, China;Department of Zoology, Shaheed Benazir Bhutto University, Sheringal, Pakistan;Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China;The Key Laboratory of New Veterinary Drug Research and Development of Jilin Province, Jilin Agricultural University, Changchun, China;The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Changchun, China;Liaoyuan Animal Disease Prevention and Control Center, Liaoyuan, China;

关键词: Enterococcus faecalis;    multidrug-resistant;    whole genome sequencing;    mobile genetic elements;    transposon;   

DOI  :  10.3389/fmicb.2023.1191837

 received in 2023-03-22, accepted in 2023-07-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Multidrug-resistant Enterococcus faecalis (E. faecalis) often cause intestinal infections in cats. The aim of this study was to investigate a multidrug-resistant E. faecalis isolate for plasmidic and chromosomal antimicrobial resistance and their genetic environment. E. faecalis strain ESC1 was obtained from the feces of a cat. Antimicrobial susceptibility testing was carried out using the broth microdilution method. Conjugation experiments were performed using Escherichia coli and Staphylococcus aureus as receptors. Complete sequences of chromosomal DNA and plasmids were generated by whole genome sequencing (WGS) and bioinformatics analysis for the presence of drug resistance genes and mobile elements. Multidrug-resistant E. faecalis ESC1 contained a chromosome and three plasmids. The amino acid at position 80 of the parC gene on the chromosome was mutated from serine to isoleucine, and hence the amino acid mutation at this site led to the resistance of ESC1 strain to fluoroquinolones. Eleven antibiotic resistance genes were located on two plasmids. We identified a novel composite transposon carrying two aminoglycoside resistance genes aac(6′)-aph(2″). This study reported the coexistence of a novel 5.4 kb composite transposon and a resistance plasmid with multiple homologous recombination in an isolate of E. faecalis ESC1. This data provides a basis for understanding the genomic signature and antimicrobial resistance mechanisms of this pathogen.

【 授权许可】

Unknown   
Copyright © 2023 Li, Qi, Li, Xue, Li, Muhammad, Li, Zhu, Ma, Kong and Ma.