期刊论文详细信息
Frontiers in Microbiology
DNA repair enzymes of the Antarctic Dry Valley metagenome
Microbiology
Ronja Stelzer1  Elizabeth Rzoska-Smith1  Adele Williamson1  Maria Monterio2  Stephen C. Cary2 
[1] Proteins and Microbes Laboratory, School of Science, University of Waikato, Hamilton, New Zealand;Thermophile Research Unit, School of Science, University of Waikato, Hamilton, New Zealand;
关键词: Antarctic Dry Valleys;    metagenome;    sequence similarity network;    DNA repair;    nuclease;    DNA ligase;   
DOI  :  10.3389/fmicb.2023.1156817
 received in 2023-02-01, accepted in 2023-03-23,  发布年份 2023
来源: Frontiers
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【 摘 要 】

Microbiota inhabiting the Dry Valleys of Antarctica are subjected to multiple stressors that can damage deoxyribonucleic acid (DNA) such as desiccation, high ultraviolet light (UV) and multiple freeze-thaw cycles. To identify novel or highly-divergent DNA-processing enzymes that may enable effective DNA repair, we have sequenced metagenomes from 30 sample-sites which are part of the most extensive Antarctic biodiversity survey undertaken to date. We then used these to construct wide-ranging sequence similarity networks from protein-coding sequences and identified candidate genes involved in specialized repair processes including unique nucleases as well as a diverse range of adenosine triphosphate (ATP) -dependent DNA ligases implicated in stationary-phase DNA repair processes. In one of the first direct investigations of enzyme function from these unique samples, we have heterologously expressed and assayed a number of these enzymes, providing insight into the mechanisms that may enable resident microbes to survive these threats to their genomic integrity.

【 授权许可】

Unknown   
Copyright © 2023 Rzoska-Smith, Stelzer, Monterio, Cary and Williamson.

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