Highlighting of the interactions of MYD88 and NFKB1 SNPs in rats resistant to decompression sickness: toward an autoimmune response

Frontiers in Physiology

Physiology

Jean-Jacques Risso¹  Anne-Virginie Desruelle¹  Nicolas Vallée¹  Céline Ramdani¹  Emmanuel Dugrenot²  François Guerrero²  Sandrine Gaillard³  Stéphane Coupé³  Simone Richard³  Régis Guieu⁴ 

[1] Institut de Recherche Biomédicale des Armées, Equipe de Recherche Subaquatique Opérationnelle, Toulon, France;Université de Brest Occidentale, ORPHY, IBSAM, Brest, France;Université de Toulon, La Garde, France;Université d’Aix-Marseille, Marseille, France;

关键词: 3′UTR;    5′UTR;    autoimmune;    decompression sickness;    dive;    hyperoxia (oxygen);   

DOI  :  10.3389/fphys.2023.1253856

 received in 2023-07-06, accepted in 2023-08-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Decompression sickness (DCS) with neurological disorders includes an inappropriate inflammatory response which degenerates slowly, even after the disappearance of the bubbles. There is high inter-individual variability in terms of the occurrence of DCS that could have been mastered by the selection and then the breeding of DCS-resistant rats. We hypothesized the selection of single-nucleotide polymorphisms (SNPs) linked to autoimmunity operated upon a generation of a DCS-resistant strain of rats. We used the candidate gene approach and targeted SNPs linked to the signaling cascade that directly regulates inflammation of innate immunity transiting by the Toll-like receptors. Twenty candidate SNPs were investigated in 36 standard rats and 33 DCS-resistant rats. For the first time, we identify a diplotype (i.e., with matched haplotypes)—when coinherited—that strengthens protection against DCS, which is not strictly homozygous and suggests that a certain tolerance may be considered. We deduced an ideal haplotype of six variants from it (MyD88_50-T, _49-A, _97-C coupled to NFKB_85-T, _69-T, _45-T) linked to the resistant phenotype. Four among the six identified variants are located in pre- and/or post-transcriptional areas regulating MyD88 or NFKB1 expression. Because of missense mutations, the other two variants induce a structural change in the NFKB1 protein complex including one damage alteration according to the Missense3D algorithm. In addition to the MyD88/NFKB1 haplotype providing rats with a strong resistance to DCS, this also highlights the importance that the immune response, here linked to the genetic heritage, can have in the development of DCS and offer a new perspective for therapeutic strategies.

【 授权许可】

Unknown   
Copyright © 2023 Vallée, Dugrenot, Desruelle, Richard, Coupé, Ramdani, Guieu, Risso, Gaillard and Guerrero.