Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

Frontiers in Plant Science

Plant Science

Lei Zhang¹  Weihu Wang¹  Jiekun Li¹  Qian Wu¹  Guoyu Hu¹  Zhiping Huang¹  Yong Yang¹  Dagang Wang¹  Yanan Wang¹ 

[1] Key Laboratory of Crop Quality Improvement of Anhui Province, Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China;

关键词: soybean;    cytoplasmic male sterility;    uninucleate microspore;    binucleate pollen;    transcriptome;    proteome;   

DOI  :  10.3389/fpls.2023.1098125

 received in 2022-11-14, accepted in 2023-01-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionHeterosis is a critical phenomenon in crop improvement. Cytoplasmic male sterility (CMS) and Restorer gene (Rf) systems are essential components for heterosis-based breeding. However, the molecular mechanism underlying CMS remains largely unclear in soybean.MethodsWe integrated a morphological investigation with comparative analyses of transcriptomic and proteomic changes in pollen from the CMS line W931A and its maintainer line, W931B, at the uninucleate microspore (UM) and binucleate pollen (BP) stages.ResultsCompared to W931B, which had healthy, oval pollen grains, W931A showed shrunken or degraded pollen grains with an irregularly thickened endothelium and decreased starch accumulation. Transcriptomic comparisons revealed a total of 865 differentially expressed genes (DEGs) in W931A over the two stages. These genes were primarily associated with pentose and glucuronate interconversions, sphingolipid metabolism, and glycerolipid metabolism. Proteomic analysis revealed 343 differentially expressed proteins (DEPs), which were mainly involved in carbon metabolism, glycolysis/gluconeogenesis, and nitrogen metabolism. Consistently, Gene Ontology (GO) biological process terms related to pollen development were enriched among DEGs at the UM and BP stages. Notably, four genes with demonstrated roles in pollen development were differentially expressed, including AGAMOUS-LIKE 104, PROTEIN-TYROSINE-PHOSPHATASE 1, and PHOSPHOLIPASE A2. A total of 53 genes and the corresponding proteins were differentially expressed in W931A at both the UM and BP stages, and many of these were pectinesterases, polygalacturonases, peroxidases, and ATPases.DiscussionThe results of this study suggest that pollen development in W931A is likely regulated through suppression of the identified DEGs and DEPs. These findings increase our understanding of the molecular mechanism underlying CMS in soybean, aiding future research into soybean fertility and promoting the efficient use of heterosis for soybean improvement.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Wang, Zhang, Yang, Wu, Hu, Wang, Li and Huang