Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.

International Journal of Molecular Sciences

Zhiming Wu²  Jiaowen Cheng³  Cheng Qin¹  Zhiqun Hu³  Caixia Yin² 

[1] Zunyi Institute of Agricultural Sciences, Zunyi 563102, Guizhou, China; E-Mail:;College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Zhongkai Road 501, Guangzhou 510225, Guangdong, China; E-Mails:;College of Horticulture, South China Agricultural University, Wushan Road 483, Guangzhou 510640, Guangdong, China; E-Mails:

关键词: Capsicum annuum L.;    cytoplasmic male sterility;    anther proteome;    two-dimensional gel electrophoresis;   

DOI  :  10.3390/ijms141122982

来源: mdpi

【 摘 要 】

Cytoplasmic male sterility (CMS), widely used in the production of hybrid seeds, is a maternally inherited trait resulting in a failure to produce functional pollen. In order to identify some specific proteins associated with CMS in pepper, two-dimensional gel electrophoresis (2-DE) was applied to proteomic analysis of anthers/buds between a CMS line (designated NA3) and its maintainer (designated NB3) in Capsicum annuum L. Thirty-three spots showed more than 1.5-fold in either CMS or its maintainer. Based on mass spectrometry, 27 spots representing 23 distinct proteins in these 33 spots were identified. Proteins down-regulated in CMS anthers/buds includes ATP synthase D chain, formate dehydrogenase, alpha-mannosidas, RuBisCO large subunit-binding protein subunit beta, chloroplast manganese stabilizing protein-II, glutathione S-transferase, adenosine kinase isoform 1T-like protein, putative DNA repair protein RAD23-4, putative caffeoyl-CoA 3-O-methyltransferase, glutamine synthetase (GS), annexin Cap32, glutelin, allene oxide cyclase, etc. In CMS anthers/buds, polyphenol oxidase, ATP synthase subunit beta, and actin are up-regulated. It was predicted that male sterility in NA3 might be related to energy metabolism turbulence, excessive ethylene synthesis, and suffocation of starch synthesis. The present study lays a foundation for future investigations of gene functions associated with pollen development and cytoplasmic male sterility, and explores the molecular mechanism of CMS in pepper.

【 授权许可】

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