Transfection of clMagR/clCry4 imparts MR-T2 imaging contrast properties to living organisms (E. coli) in the presence of Fe3+ by endogenous formation of iron oxide nanoparticles

Frontiers in Molecular Biosciences

Molecular Biosciences

Yuanyuan Xie¹  Bin Wang¹  Xiaoli Mai²  Chenzhuo Zhu³  Xiaofeng Han⁴  Le Xue⁵  Jianfei Sun⁵  Nuan Li⁵  Peng Wang⁶  Yuqing Ge⁷  Yewei Zhang⁸ 

[1] Clinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China;Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China;Southeast University-Monash University Joint Graduate School, Southeast University, Suzhou, China;State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China;State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China;Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China;State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, China;Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China;Department of Sports Medicine and Adult Reconstructive Surgery, The Affiliated Drum Tower Hospital of Nanjing University, Nanjing, China;State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, China;The Hepatopancreatobiliary Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China;

关键词: contrast agent;    iron oxide nanoparticles;    biosynthesis;    MRI;    clMagR/clCry4;   

DOI  :  10.3389/fmolb.2023.1119356

 received in 2022-12-08, accepted in 2023-01-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Rapid development of medical imaging, such as cellular tracking, has increased the demand for “live” contrast agents. This study provides the first experimental evidence demonstrating that transfection of the clMagR/clCry4 gene can impart magnetic resonance imaging (MRI) T2-contrast properties to living prokaryotic Escherichia coli (E. coli) in the presence of Fe3+ through the endogenous formation of iron oxide nanoparticles. The transfected clMagR/clCry4 gene markedly promoted uptake of exogenous iron by E. coli, achieving an intracellular co-precipitation condition and formation of iron oxide nanoparticles. This study will stimulate further exploration of the biological applications of clMagR/clCry4 in imaging studies.

【 授权许可】

Unknown   
Copyright © 2023 Li, Xue, Mai, Wang, Zhu, Han, Xie, Wang, Ge, Zhang and Sun.