Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota

Frontiers in Immunology

Immunology

Bin Feng¹  Ji Chen²  Xing-Fu Chen²  Meng-Liang Tian²  Chao Huang³  Zhong-Qiong Yin⁴  Xiao-Ping JiZe⁴  Xing-Hong Zhao⁴  Chao-Wen Zhang⁴  Li-Xia Li⁴  Hua-Qiao Tang⁴  Yu-Ping Fu⁴  Cen-Yu Li⁴  Ning-Yuan Li⁴  Xu Song⁴  Gang Ye⁴  Yuan-Feng Zou⁵  Yang-Ping Li⁶ 

[1] Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China;College of Agronomy, Sichuan Agricultural University, Chengdu, China;Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China;Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China;Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China;Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China;State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, China College of Agronomy, Sichuan Agricultural University, Chengdu, China;

关键词: Chuanminshen violaceum;    polysaccharide;    antioxidation;    antiinflammatory;    gut-liver axis;    gut microbiota;   

DOI  :  10.3389/fimmu.2023.1159291

 received in 2023-02-05, accepted in 2023-03-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo.

【 授权许可】

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Copyright © 2023 Zou, JiZe, Li, Zhang, Fu, Yin, Li, Song, Li, Zhao, Feng, Huang, Ye, Tang, Li, Chen, Chen and Tian