Investigating the microbiome of house dust mites in South Korea

Frontiers in Allergy

Allergy

Ju Yeong Kim¹  Tai-Soon Yong²  Myung-hee Yi²  Myungjun Kim² 

[1] Correspondence:;Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea;

关键词: house dust mites;    microbiome;    Dermatophagoides farinae;    16S rDNA;    Bartonella;    Enterococcus;   

DOI  :  10.3389/falgy.2023.1240727

 received in 2023-06-15, accepted in 2023-08-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

Understanding the house dust mites (HDMs) microbiome is crucial due to its potential effects on the development of allergic diseases. In 1998, our laboratory collected Dermatophagoides farinae and D. pteronyssinus from beds in a Korean household and began cultivating these HDMs. Our laboratory has been actively investigating several topics about HDMs in recent years, including the bacterial and fungal microbiome and their interactions, as well as the impact of the HDM microbiome on airway inflammation. To study the D. farinae microbiome, we employed high-throughput sequencing of the 16S rDNA amplicons. The results revealed that the two most abundant bacteria were Enterococcus faecalis and Bartonella spp. In contrast, we found almost no bacteria in D. pteronyssinus. By inoculating bacteria to HDMs, we found that D. farinae is more susceptible to bacteria than D. pteronyssinus. This susceptibility was associated with the presence of certain fungal species in D. pteronyssinus. Additionally, we have recently made efforts to produce HDMs with reduced levels of symbiotic bacteria. We believe that standardizing and controlling the microbiome in HDMs are crucial steps for the future development and improvement of allergic immunotherapies.

【 授权许可】

Unknown   
© 2023 Yi, Kim, Yong and Kim.