Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury

Frontiers in Molecular Neuroscience

Molecular Neuroscience

Qingying Si¹  Xue Chu²  Junjun Meng²  Yanan Li²  Jinxiu Guo²  Pei Jiang³  Xin Xie⁴ 

[1] Department of Endocrinology, Tengzhou Central People's Hospital, Tengzhou, China;Translational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, China;Translational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, China;Institute of Translational Pharmacy, Jining Medical Research Academy, Jining, China;Translational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, China;School of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, China;

关键词: spontaneously hypertensive rats;    hypertension;    metabolomics;    transcriptomics;    hippocampus;   

DOI  :  10.3389/fnmol.2023.1146525

 received in 2023-01-17, accepted in 2023-03-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveHypertension is a public health challenge worldwide due to its high prevalence and multiple complications. Hypertension-induced damage to the hippocampus leads to behavioral changes and various brain diseases. Despite the multifaceted effects of hypertension on the hippocampus, the mechanisms underlying hippocampal lesions are still unclear.MethodsThe 32-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were selected as the study subjects. Behavioral experiments such as an open field test (OFT), an elevated plus maze (EPM) test, and the Morris water maze (MWM) test were performed to show the behavioral characteristics of the rats. A comprehensive transcriptomic and metabolomic analysis was performed to understand the changes in the hippocampus at the metabolic and genetic levels.ResultsBehavioral tests showed that, compared to WKY rats, SHR showed not only reduced memory capacity but more hyperactive and impulsive behavior. In addition, transcriptomic analysis screened for 103 differentially expressed genes. Metabolomic analysis screened 56 metabolites with significant differences, including various amino acids and their related metabolites.ConclusionComprehensive analysis showed that hypertension-induced hippocampal lesions are closely associated with differential metabolites and differential genes detected in this study. The results provide a basis for analyzing the mechanisms of hypertension-induced hippocampal damage.

【 授权许可】

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Copyright © 2023 Li, Chu, Xie, Guo, Meng, Si and Jiang.