Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats

Proteome Science

Research

Hasni Misbah¹  See Z Hoe²  Ruby Husain²  Nur-Atiqah H Abdullah³  Saiful A Karsani⁴  Norhaniza Aminudin⁴  Onn H Hashim⁵ 

[1] Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;University of Malaya Centre for Proteomics Research, University of Malaya, Kuala Lumpur, Malaysia;University of Malaya Centre for Proteomics Research, University of Malaya, Kuala Lumpur, Malaysia;Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;

关键词: hypertension;    biomarker;    spontaneously hypertensive rats;    serum proteins;    proteomics;   

DOI  :  10.1186/1477-5956-10-17

 received in 2011-08-17, accepted in 2012-03-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundProteins that are associated with hypertension may be identified by comparing the 2-dimensional gel electrophoresis (2-DE) profiles of the sera of spontaneously hypertensive rats (SHR) with those generated from normotensive Spraque-Dawley rats (SDR).ResultsFive proteins of high abundance were found to be significantly altered when the 2-DE serum profiles of the SHR were compared to those that were similarly generated from the SDR. Analysis by mass spectrometry and database search identified the proteins as retinol binding protein 4, complement C3, albumin (19.9 kDa fragment), alpha1 macroglobulin and alpha1 antiproteinase, which are all known to be associated with hypertension. The altered expression of the two latter proteins was found to be abrogated when similar analysis was performed on sera of the SHR that were treated with captopril.ConclusionOur data suggests that serum alpha1 macroglobulin and alpha1 antiproteinase are potentially useful complementary biomolecular indicators for monitoring of hypertension.

【 授权许可】

CC BY   
© Aminudin et al; licensee BioMed Central Ltd. 2012