Development of proteomic techniques for biomarker discovery

QH301 Biology;QD Chemistry

Allingham, Heather ; Pitt, A.R.

University:University of Glasgow

Department:Institute of Molecular Cell and Systems Biology

关键词: proteomics, biomarker identification, mass spectrometry, label free quantitation, hypertension;   

Others  :  http://theses.gla.ac.uk/3145/1/2012allinghamphd.pdf

来源: University of Glasgow

【 摘 要 】

The main aim of my research, presented here, is to develop proteomic research techniques, for their use in biomarker discovery and identification. This is broken down into three main chapters:•Biomarker Identification in Stroke Brain guided by MALDI-imaging.•Evaluation of the Effectiveness of Heat Treatment for Prevention of Proteomic Sample Degradation using Label Free Relative Quantitation.•Discovery and Identification of Biomarkers for Hypertension Within these chapters special attention is paid to sample preparation, development and assessment of new methods for biomarkers discovery and identification, the importance of experimental design and the application of relevant and useful statistical methods to enable the mining of useful information from rich datasets. The biological changes in stroke induced mouse brain tissue are studied, the prevention of degradation to tissue samples by a novel heat treatment method and changes to plasma samples from hypertensive, wild type and a congenic strain of rat are also studied. The outcomes of this work are multiple, namely:•The identification of a possible marker for stroke from mouse brain tissue•The effect of a new heat treatment device on proteomic data obtained from mouse brain tissue•The novel application of a statistical analysis to a new type of dataset (LC-MS and label free quantitation of biological samples)•The identification of possible biomarkers for hypertension in rat plasma using this method