Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains

Frontiers in Microbiology

Microbiology

Jingjing Zhang¹  Tianjie Sun¹  Zina Zhang¹  Dongdong Zhang¹  Yinbo Bian¹  Gangyi Zhao¹  Chunyan Hou¹  Shengfang Han¹  Dongmei Wang¹ 

[1] State Key Laboratory of North China Crop Improvement and Regulation, Baoding, China;Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding, China;College of Life Sciences, Hebei Agricultural University, Baoding, China;

关键词: take-all disease;    Bacillus subtilis;    biological control;    combination of strains;    antimicrobial protein;   

DOI  :  10.3389/fmicb.2023.1118176

 received in 2022-12-07, accepted in 2023-01-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Wheat (Triticum aestivum) is the second largest grain crop worldwide, and one of the three major grain crops produced in China. Take-all disease, caused by Gaeumannomyces graminis var. tritici (Ggt) infection, is a widespread and devastating soil-borne disease that harms wheat production. At present, the prevention and control of wheat take-all depend largely on the application of chemical pesticides. Chemical pesticides, however, not only lead to increased drug resistance of pathogens but also leave significant residues in the soil, causing serious environmental pollution. In this study, we investigated the application of Bacillus subtilis to achieve take-all disease control in wheat while reducing pesticide application. Antagonistic bacteria were screened by plate test, species identification of strains was performed by Gram staining and sequencing of 16s rDNA, secondary metabolite activity of strains was detected by clear circle method, strain compatibility and effect of compounding on Ggt were detected by plate, and the application prospects of specific strains were analyzed by greenhouse and field experiments. We found that five B. subtilis strains, JY122, JY214, ZY133, NW03, Z-14, had significant antagonistic effects against Ggt, and could secrete antimicrobial proteins including amylase, protease, and cellulase. Furthermore, Z-14 and JY214 cultures have also been shown to change the morphology of Ggt mycelium. These results also showed that Z-14, JY214, and their combination can control take-all disease in wheat at a reduced level of pesticide use. In summary, we screened two Bacillus spp. strains, Z-14 and JY214, that could act as antagonists that contribute to the biological control of wheat take-all disease. These findings provide resources and ideas for controlling crop diseases in an environmentally friendly manner.

【 授权许可】

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Copyright © 2023 Zhao, Sun, Zhang, Zhang, Bian, Hou, Zhang, Han and Wang.