Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916

Journal of Integrative Agriculture

Dong-mei SUN¹  Tan-tan GAO²  Qi PENG³  Ya-jing XIAO⁴  Jie ZHANG⁵  Fu-ping SONG⁵ 

[1] GAO Tan-tan, Tel: [phone];State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China;XIAO Ya-jing, Tel: [phone];College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R. China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China;

关键词: methylerythritol phosphate pathway;    transcription;    Bacillus subtilis;    biological control;   

DOI  :  

来源: DOAJ

【 摘 要 】

The methylerythritol phosphate pathway is responsible for the biosynthesis of terpenoids, the largest class of secondary metabolites. Although the structures and functions of the proteins involved in this pathway have been well studied in Bacillus subtilis, only a few studies have reported the transcriptional profile of the genes involved. Therefore, we analyzed methylerythritol phosphate pathway genes in the genome of B. subtilis 916, which has been developed as a biological control agent against some rice diseases in China. Our results showed that methylerythritol phosphate pathway genes were distributed throughout the genome of this strain. These genes were transcribed during both the exponential and stationary phases. We further confirmed the transcription units of dxs, dxr, ispD, ispF, ipK, ispG, ispH, idi, and ispA in B. subtilis 916 through reverse transcription-PCR analyses; the results showed that these nine genes were located in seven different operons. The transcript start sites of the seven different operons were determined by 5’-rapid amplification of cDNA ends-PCR. Thus, our study provides a molecular basis at the transcriptional level for investigating homoterpene synthesis in the methylerythritol phosphate pathway of B. subtilis 916.

【 授权许可】

Unknown