A signaling complex of adenylate cyclase CyaC of Sinorhizobium meliloti with cAMP and the transcriptional regulators Clr and CycR

BMC Microbiology

Research

Gottfried Unden¹  Robin Klein¹  Juliane Wissig¹  Jannis Brehm¹  Ralf Heermann¹ 

[1] Institute of Molecular Physiology (imP), Microbiology and Biotechnology, Johannes Gutenberg University, Biocenter II, Hanns-Dieter-Hüsch-Weg 17, 55128, Mainz, Germany;

关键词: Second messenger;    CyaC;    Cyclic AMP;    TetR-type regulator;    cyclic-AMP-receptor protein CRP;    Sinorhizobium meliloti;    Ensifer meliloti;   

DOI  :  10.1186/s12866-023-02989-5

 received in 2023-05-29, accepted in 2023-08-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAdenylate cyclases (ACs) generate the second messenger cyclic AMP (cAMP), which is found in all domains of life and is involved in the regulation of various cell physiological and metabolic processes. In the plant symbiotic bacterium Sinorhizobium meliloti, synthesis of cAMP by the membrane-bound AC CyaC responds to the redox state of the respiratory chain and the respiratory quinones. However, nothing is known about the signaling cascade that is initiated by cAMP produced by CyaC.ResultsHere, the CRP-like transcriptional regulator Clr and the TetR-like regulator CycR (TR01819 protein) were identified to interact with CyaC using the bacterial two-hybrid system (BACTH), co-sedimentation assays, and surface plasmon resonance spectroscopy. Interaction of CycR with Clr, and of CyaC with Clr requires the presence of cAMP and of ATP, respectively, whereas that of CyaC with CycR was independent of the nucleotides.ConclusionThe data implicate a ternary CyaC×CycR×cAMP-Clr complex, functioning as a specific signaling cascade which is formed after activation of CyaC and synthesis of cAMP. cAMP-Clr is thought to work in complex with CycR to regulate a subset of genes of the cAMP-Clr regulon in S. meliloti.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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