Lactiplantibacillus plantarum, lactiplantibacillus pentosus and inulin meal inclusion boost the metagenomic function of broiler chickens

Animal Microbiome

Research

Sihem Dabbou¹  Ilaria Biasato²  Kalliopi Rantsiou²  Luca Cocolin²  Ilario Ferrocino²  Laura Gasco²  Simone Squara³  Erica Liberto³  Chiara Emilia Cordero³  Elena Colombino⁴  Achille Schiavone⁴  Maria Teresa Capucchio⁴  Marta Gariglio⁴ 

[1] Center Agriculture Food Environment (C3A), University of Trento, Turin, Italy;Department of Agricultural, Forest and Food Sciences, University of Turin, Turin, Italy;Department of Drug Science and Technology, University of Turin, Turin, Italy;Department of Veterinary Sciences, University of Turin, Turin, Italy;

关键词: Lactic acid bacteria;    Probiotic;    Prebiotic;    Inulin;    microbiota;    Broiler chickens;   

DOI  :  10.1186/s42523-023-00257-5

 received in 2023-01-31, accepted in 2023-07-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe inclusion of alternative ingredients in poultry feed is foreseen to impact poultry gut microbiota. New feeding strategies (probiotics/prebiotics) must be adopted to allow sustainable productions. Therefore, the current study aimed to use metagenomics approaches to determine how dietary inclusion of prebiotic (inulin) plus a multi-strain probiotic mixture of Lactiplantibacillus plantarum and Lactiplantibacillus pentosus affected microbiota composition and functions of the gastro-intestinal tract of the broilers during production. Fecal samples were collected at the beginning of the trial and after 5, 11 and 32 days for metataxonomic analysis. At the end of the trial, broilers were submitted to anatomo-pathological investigations and caecal content was subjected to volatilome analysis and DNAseq.ResultsProbiotic plus prebiotic inclusion did not significantly influence bird performance and did not produce histopathological alterations or changes in blood measurements, which indicates that the probiotic did not impair the overall health status of the birds. The multi-strain probiotic plus inulin inclusion in broilers increased the abundance of Blautia, Faecalibacterium and Lachnospiraceae and as a consequence an increased level of butyric acid was observed. In addition, the administration of probiotics plus inulin modified the gut microbiota composition also at strain level since probiotics alone or in combination with inulin select specific Faecalibacterium prausnitzi strain populations. The metagenomic analysis showed in probiotic plus prebiotic fed broilers a higher number of genes required for branched-chain amino acid biosynthesis belonging to selected F. prausnitzi strains, which are crucial in increasing immune function resistance to pathogens. In the presence of the probiotic/prebiotic a reduction in the occurrence of antibiotic resistance genes belonging to aminoglycoside, beta-lactamase and lincosamide family was observed.ConclusionsThe positive microbiome modulation observed is particularly relevant, since the use of these alternative ingredients could promote a healthier status of the broiler’s gut.

【 授权许可】

CC BY   
© BioMed Central Ltd. 2023

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