Modulation of the human gut microbiota by dietary fibres occurs at the species level

BMC Biology

Research Article

Joan Vermeiren¹  Douwina Bosscher¹  Petra Louis²  Sylvia H. Duncan²  Harry J. Flint²  Wing Sun Faith Chung²  Alan W. Walker³  Julian Parkhill⁴ 

[1] Cargill R&D Centre Europe, Vilvoorde, Belgium;Microbiology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK;Microbiology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK;Pathogen Genomics Group, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, Cambridgeshire, UK;Pathogen Genomics Group, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, Cambridgeshire, UK;

关键词: Bacteroidetes;    Prebiotic;    Colonic anaerobes;    Faecalibacterium prausnitzii;    Firmicutes;    Inulin;    Pectin;    Propionate;   

DOI  :  10.1186/s12915-015-0224-3

 received in 2015-09-21, accepted in 2015-12-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDietary intake of specific non-digestible carbohydrates (including prebiotics) is increasingly seen as a highly effective approach for manipulating the composition and activities of the human gut microbiota to benefit health. Nevertheless, surprisingly little is known about the global response of the microbial community to particular carbohydrates. Recent in vivo dietary studies have demonstrated that the species composition of the human faecal microbiota is influenced by dietary intake. There is now potential to gain insights into the mechanisms involved by using in vitro systems that produce highly controlled conditions of pH and substrate supply.ResultsWe supplied two alternative non-digestible polysaccharides as energy sources to three different human gut microbial communities in anaerobic, pH-controlled continuous-flow fermentors. Community analysis showed that supply of apple pectin or inulin resulted in the highly specific enrichment of particular bacterial operational taxonomic units (OTUs; based on 16S rRNA gene sequences). Of the eight most abundant Bacteroides OTUs detected, two were promoted specifically by inulin and six by pectin. Among the Firmicutes, Eubacterium eligens in particular was strongly promoted by pectin, while several species were stimulated by inulin. Responses were influenced by pH, which was stepped up, and down, between 5.5, 6.0, 6.4 and 6.9 in parallel vessels within each experiment. In particular, several experiments involving downshifts to pH 5.5 resulted in Faecalibacterium prausnitzii replacing Bacteroides spp. as the dominant sequences observed. Community diversity was greater in the pectin-fed than in the inulin-fed fermentors, presumably reflecting the differing complexity of the two substrates.ConclusionsWe have shown that particular non-digestible dietary carbohydrates have enormous potential for modifying the gut microbiota, but these modifications occur at the level of individual strains and species and are not easily predicted a priori. Furthermore, the gut environment, especially pH, plays a key role in determining the outcome of interspecies competition. This makes it crucial to put greater effort into identifying the range of bacteria that may be stimulated by a given prebiotic approach. Both for reasons of efficacy and of safety, the development of prebiotics intended to benefit human health has to take account of the highly individual species profiles that may result.

【 授权许可】

CC BY   
© Chung et al. 2016