Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion

AMB Express

Original Article

Dongliang Li¹  Fang Xue¹  Cheng Luo¹  Youran Li²  Xufan Ma²  Guiyang Shi² 

[1] Key Laboratory of Chinese Cigar Fermentation, Cigar Technology Innovation Center of China Tobacco, Tobacco Sichuan Industrial Co., Ltd, 610000, Chengdu, P. R. China;National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, Jiangsu, P. R. China;Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, Jiangsu, P. R. China;

关键词: Bacillus licheniformis;    Homologous recombination;    Recombinase RecT;   

DOI  :  10.1186/s13568-023-01589-w

 received in 2023-06-10, accepted in 2023-07-29,  发布年份 2023

来源: Springer

【 摘 要 】

Bacillus licheniformis and its related strains have found extensive applications in diverse industries, agriculture, and medicine. However, the current breeding methods for this strain primarily rely on natural screening and traditional mutagenesis. The limited availability of efficient genetic engineering tools, particularly recombination techniques, has hindered further advancements in its applications. In this study, we conducted a comprehensive investigation to identify and characterize a recombinase, RecT, derived from a Bacillus phage. Remarkably, the recombinase exhibited a 105-fold enhancement in the recombination efficiency of the strain. To facilitate genome editing, we developed a system based on the conditional expression of RecT using a rhamnose-inducible promoter (Prha). The efficacy of this system was evaluated by deleting the amyL gene, which encodes an α-amylase. Our findings revealed that the induction time and concentration of rhamnose, along with the generation time of the strain, significantly influenced the editing efficiency. Optimal conditions for genome editing were determined as follows: the wild-type strain was initially transformed with the genome editing plasmid, followed by cultivation and induction with 1.5% rhamnose for 8 h. Subsequently, the strain was further cultured for an additional 24 h, equivalent to approximately three generations. Consequently, the recombination efficiency reached an impressive 16.67%. This study represents a significant advancement in enhancing the recombination efficiency of B. licheniformis through the utilization of a RecT-based recombination system. Moreover, it provides a highly effective genome editing tool for genetic engineering applications in this strain.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

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