Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems

BMC Veterinary Research

Research

Virginia Aragon¹  Xiao Hu²  Nubia R. Macedo²  Orhan Sahin²  Rodger Main²  Ganwu Li²  Robert Mugabi²  Ana Paula S. Poeta Silva²  Maria J. Clavijo³  Alexander W. Tucker⁴  Marcelo Gottschalk⁵  Perry Harms⁶ 

[1] Centre de Recerca en Sanitat Animal (CReSA), Unitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, UniversitatAutònoma de Barcelona (UAB), Campus, Bellaterra, Barcelona, Spain;Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), IRTA, UniversitatAutònoma de Barcelona (UAB), Campus, Bellaterra, Barcelona, Spain;WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), Bellaterra, Barcelona, Spain;Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA;Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA;PIC North America, Hendersonville, TN, USA;Department of Veterinary Medicine, University of Cambridge, CB3 0ES, Cambridge, UK;Groupe de Recherche Sur Les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Montréal, Canada;PIC North America, Hendersonville, TN, USA;

关键词: Glaesserella parasuis;    Swine;    Polyserositis;    Serotyping;    Whole-genome sequencing;    Multilocus sequence typing;    Phylogeny;   

DOI  :  10.1186/s12917-023-03698-x

 received in 2023-03-24, accepted in 2023-08-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundGlaesserella parasuis is the causative agent of Glässer’s disease in pigs. Serotyping is the most common method used to type G. parasuis isolates. However, the high number of non-typables (NT) and low discriminatory power make serotyping problematic. In this study, 218 field clinical isolates and 15 G. parasuis reference strains were whole-genome sequenced (WGS). Multilocus sequence types (MLST), serotypes, core-genome phylogeny, antimicrobial resistance (AMR) genes, and putative virulence gene information was extracted.ResultsIn silico WGS serotyping identified 11 of 15 serotypes. The most frequently detected serotypes were 7, 13, 4, and 2. MLST identified 72 sequence types (STs), of which 66 were novel. The most predominant ST wasST454. Core-genome phylogeny depicted 3 primary lineages (LI, LII, and LIII), with LIIIA sublineage isolates lacking all vtaA genes, based on the structure of the phylogenetic tree and the number of virulence genes. At least one group 1 vtaA virulence genes were observed in most isolates (97.2%), except for serotype 8 (ST299 and ST406), 15 (ST408 and ST552) and NT (ST448). A few group 1 vtaA genes were significantly associated with certain serotypes or STs. The putative virulence gene lsgB, was detected in 8.3% of the isolates which were predominantly of serotype 5/12. While most isolates carried the bcr, ksgA, and bacA genes, the following antimicrobial resistant genes were detected in lower frequency;  blaZ (6.9%), tetM (3.7%), spc (3.7%), tetB (2.8%), bla-ROB-1 (1.8%), ermA (1.8%), strA (1.4%), qnrB (0.5%), and aph3''Ia (0.5%).  ConclusionThis study showed the use of WGS to type G. parasuis isolates and can be considered an alternative to the more labor-intensive and traditional serotyping and standard MLST. Core-genome phylogeny provided the best strain discrimination. These findings will lead to a better understanding of the molecular epidemiology and virulence in G. parasuis that can be applied to the future development of diagnostic tools, autogenous vaccines, evaluation of antibiotic use, prevention, and disease control.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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