Variations in diversity, composition, and species interactions of soil microbial community in response to increased N deposition and precipitation intensity in a temperate grassland

Ecological Processes

Research

Pengfeng Wu¹  Guo Zheng¹  Shuyan Cui¹  Yushan Xiao¹  Yu Zhou¹  Liqiang Cui² 

[1] College of Life Science, Shenyang Normal University, 110034, Shenyang, China;School of Environmental Science and Engineering, Yancheng Institute of Technology, 224051, Yancheng, China;

关键词: Microbial community composition;    Phospholipid fatty acid;    High-throughput sequencing;    Precipitation intensity;    N deposition;   

DOI  :  10.1186/s13717-023-00445-w

 received in 2023-05-08, accepted in 2023-07-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundGlobal climate change has resulted in precipitation regimes exhibiting an increasing trend in rainfall intensity but a reduction in frequency. In addition, nitrogen (N) deposition occurs simultaneously in arid and semi-arid regions. Microbial biomass, diversity, composition, and species interactions are key determinants of ecological functions. We examined the effects of changes in precipitation intensity and N addition on the soil bacterial and fungal communities in a semi-arid grassland in Inner Mongolia, China.MethodsThe microbial biomass (bacterial PLFAs and fungal PLFAs) was determined through phospholipid fatty acid (PLFA) analysis, and microbial diversity (Shannon index and evenness index) was determined with high-throughput sequencing (16S and ITS). Species interactions were determined using a molecular ecological network analysis. The relationships between microbial community (bacterial community and fungal community) and environmental variables were examined by Mantel tests.ResultsWe found that N addition decreased fungal PLFA under moderate, high, and extreme precipitation intensity treatments and increased fungal community complexity under the high precipitation intensity treatment. Furthermore, N addition increased bacterial diversity under moderate and high precipitation intensity treatments. N addition caused greater environmental stress to the fungal community, which was dominated by deterministic processes.ConclusionsThe effects of N deposition on soil bacterial and fungal communities were altered by precipitation intensity. The changes in soil bacterial and fungal communities were different, implying that composition and functional traits adapt differently to projected global changes at a regional scale.

【 授权许可】

CC BY   
© The Author(s) 2023

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