Anti-Pathogenic Activity of Coral Bacteria Againts White Plaque Disease of Coral Dipsastraea from Tengah Island, Karimunjawa

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Muchlissin, Sakti Imam^1 ; Sabdono, Agus^2 ; Permata, W. Diah^2

Tropical Marine Biotechnology Laboratory, Diponegoro University, Semarang, Indonesia^1

Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia^2

关键词: and Molecula;    Anti-bacterial activity;    Biological controls;    Coral disease;    Dipsastraea;    Microbial community composition;    Pathogenic activity;    White Plaque;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012057/pdf
DOI  :  10.1088/1755-1315/116/1/012057

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Coral disease is main factor of degrading coral reefs, such as White Plaque (WP) disease that cause loss of epidermal tissue of corals. The purposes of this research were to identify the bacteria associated with White Plaque Disease of coral Dipsastraea and to investigate coral bacteria that have antipathogenic potency against White Plaque Disease by Coral Dipsastraea. Sampling was carried out by purposive method in Tengah Island, Karimunjawa on March 2015. Streak method was used to isolate and purify coral bacteria, while overlay and agar diffusion method were used to test antibacterial activity. Identification of selected bacteria was conducted by biochemical and molecular methods. Polyphasic identification of bacteria associated with diseased coral White Plague of Dipsastraea. It is found that TFWP1, TFWP2, TFWP3 and TFWP4 were closely related to Bacillus antracis, Virgibacillus olivae, Virgibacillus salarius and Bacillus mojavensis, respectively. While antipathogen activity bacterial isolates, NM1.3, NM1.8 and NM2.3 were closely related to Pseudoalteromonas flavipulchra, Pseudoalteromonas piscicida, and Vibrio azureus, respectively. Phylogenetic data on microbial community composition in coral will help with the knowledge in the biological control of coral diseases.