Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

BMC Microbiology

Research

Weili Zhang¹  Ling Shu¹  Xiaohan Li¹  Yuling Xiao¹  Keping Ao¹  Siying Wu¹  Mei Kang¹  Zhixing Chen¹  Ya Liu¹  Yi Xie¹ 

[1] Department of Laboratory Medicine, West China Hospital, Sichuan University, No. 37 Guoxue Lane, 610041, Chengdu, China;

关键词: MALDI-TOF;    Autof ms1000;    Mass spectrometry;    Filamentous fungi;    Isolates identification;    Accuracy;   

DOI  :  10.1186/s12866-023-02968-w

 received in 2022-08-11, accepted in 2023-08-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microbial identification. However, there is a lack of data on its performance in identifying filamentous fungi. The objective of our study was to evaluate the accuracy of the Autof ms1000 mass spectrometry for identifying filamentous fungi in the clinical microbiology laboratory.ResultsAmong 106 samples tested using the Autof ms1000 system, 101 (95.28%) were identified at the genus or species level, and 81 (76.41%) were accurately identified at the species level. Additionally, we developed a new rapid formic acid extraction method with simple pretreatment for filamentous fungi that saved time and provided accurate results.ConclusionsThe Autof ms1000 mass spectrometer proved to be a valuable tool for identifying filamentous fungi. However, upgrading the database is recommended for correctly identifying rare strains.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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