The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar

RENEWABLE ENERGY

卷:165

Article

de Lucas, Rosymar Coutinho^1,2,3  de Oliveira, Tassio Brito³  Lima, Matheus Sanita⁴  Pasin, Thiago Machado¹  de Almeida Scarcella, Ana Silvia¹  Costa Ribeiro, Liliane Fraga¹  Carvalho, Caio³  de Lima Damasio, Andre Ricardo⁵  Buckeridge, Marcos Silveira⁶  Prade, Rolf Alexander²  Segato, Fernando^2,7  Teixeira de Moraes Polizeli, Maria de Lourdes^1,3 

[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP, Brazil

[2] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

[3] Univ Sao Paulo, Dept Biol, FFCLRP, BR-14040901 Ribeirao Preto, SP, Brazil

[4] Univ Western Ontario, Dept Biol, London, ON, Canada

[5] Univ Estadual Campinas, Inst Biol, Dept Bioquim & Biol Tecidual, Campinas, SP, Brazil

[6] Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, SP, Brazil

[7] Univ Sao Paulo, Dept Biotecnol, Escola Engn Lorena, Lorena, SP, Brazil

关键词: Secretome;    Enzymes;    Filamentous fungi;    Mass spectrometry;    Agro-industrial residue;   

DOI  :  10.1016/j.renene.2020.11.072

来源: Elsevier

【 摘 要 】

The plant cell wall is the most abundant carbon reservoir in nature and is a renewable source of biofuels. To break down this biomass and convert it into fermentable sugars, a set of multiple enzymes is needed. Here, we characterize the enzymatic repertoire necessary for the degradation of sugarcane bagasse in natura and pre-treated using Aspergillus clavatus as a model. 135 unique peptides were identified by Mass Spectrometry MS/MS. 23 of these proteins belong to classes of enzymes involved in biomass degradation and were differentially expressed on various substrates. Each pretreatment changed the sugarcane bagasse composition, which, in turn, led to the differential expression of A. clavatus holocellulases. The deconstruction of in natura bagasse demanded the largest set of enzymes due to the structural complexity of this material. Not only different sources of biomass but also different pretreatments of the same source will determine the enzymes required for the most efficient biomass conversion, avoiding the use of non-essential enzymes and consequent financial expense. Understanding A. clavatus nutritional strategies by proteomic analysis of secretome can improve the technology applied to biomass conversion and by-product synthesis. (C) 2020 Published by Elsevier Ltd.

【 授权许可】

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