In vitro antibiofilm and bacteriostatic activity of diacerein against Enterococcus faecalis

AMB Express

Original Article

Chunyan Fu¹  Jiabei Cai¹  Jing Yang¹  Yuan Liu¹  Yuxi Xu¹  Peiyu Wang¹  Chengzhi Zheng¹  Leihao Tian¹  Meiqin Zheng²  Hao Zheng³  Xinyi Ling³  Yuanyuan Li³  Yongliang Lou³  Xiaobin Gu³ 

[1] Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China;National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, China;Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China;National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, China;Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China;Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China;

关键词: Enterococcus faecalis;    Biofilm;    Diacerein;    RNA-seq;    Esp;    LuxS;   

DOI  :  10.1186/s13568-023-01594-z

 received in 2023-07-29, accepted in 2023-08-04,  发布年份 2023

来源: Springer

【 摘 要 】

Enterococcus faecalis is one of the main pathogens that causes hospital-acquired infections because it is intrinsically resistant to some antibiotics and often is capable of biofilm formation, which plays a critical role in resisting the external environment. Therefore, attacking biofilms is a potential therapeutic strategy for infections caused by E. faecalis. Current research indicates that diacerein used in the treatment of osteoarthritis showed antimicrobial activity on strains of gram-positive cocci in vitro. In this study, we tested the MICs of diacerein using the broth microdilution method, and successive susceptibility testing verified that E. faecalis is unlikely to develop resistance to diacerein. In addition, we obtained a strain of E. faecalis HE01 with strong biofilm-forming ability from an eye hospital environment and demonstrated that diacerein affected the biofilm development of HE01 in a dose-dependent manner. Then, we explored the mechanism by which diacerein inhibits biofilm formation through qRT-PCR, extracellular protein assays, hydrophobicity assays and transcriptomic analysis. The results showed that biofilm formation was inhibited at the initial adhesion stage by inhibition of the expression of the esp gene, synthesis of bacterial surface proteins and reduction in cell hydrophobicity. In addition, transcriptome analysis showed that diacerein not only inhibited bacterial growth by affecting the oxidative phosphorylation process and substance transport but also inhibited biofilm formation by affecting secondary metabolism, biosynthesis, the ribosome pathway and luxS expression. Thus, our findings provide compelling evidence for the substantial therapeutic potential of diacerein against E. faecalis biofilms.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

【 图 表 】

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