Bioorthogonal Engineered Virus-Like Nanoparticles for Efficient Gene Therapy

Nano-Micro Letters

Article

Xin-Ping Feng¹  Wei Cui¹  Wan-Liang Lu²  Pei-Shan Li²  Yi-Xuan Liu²  Jin-Ling Wang²  Gui-Ling Wang²  Ying Xie²  Song-Yue Chen²  Jia-Lun Duan³  Chun-Jie Bao⁴ 

[1] School of Chemical Sciences, University of Chinese Academy of Sciences, 100049, Beijing, People’s Republic of China;State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and Drug Delivery Systems, and School of Pharmaceutical Sciences, Peking University, 100191, Beijing, People’s Republic of China;State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and Drug Delivery Systems, and School of Pharmaceutical Sciences, Peking University, 100191, Beijing, People’s Republic of China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 210023, Nanjing, People’s Republic of China;State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and Drug Delivery Systems, and School of Pharmaceutical Sciences, Peking University, 100191, Beijing, People’s Republic of China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 210023, Nanjing, People’s Republic of China;School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, 210023, Nanjing, People’s Republic of China;

关键词: Virus-like nanoparticle;    Site-specific codon mutation;    Recombinant biosome;    Bioorthogonal chemistry;    Gene therapy;   

DOI  :  10.1007/s40820-023-01153-y

 received in 2023-04-10, accepted in 2023-06-20,  发布年份 2023

来源: Springer

【 摘 要 】

tsA virus-like nanoparticle (reBiosome) was developed via site-specific codon mutation for displaying unnatural amino acid (Azi) on virus envelope protein at a rational site, followed by conjugating weak acid-responsive polyethylene glycol polymer on Azi via bioorthogonal chemistry. The reBiosome exhibited reduced virus-like immunogenicity, prolonged blood circulation and enhanced delivery to weakly acidic disease foci. The reBiosome enabled efficient delivery of gene editing and gene silencing system, demonstrating remarkable therapeutic efficacy in breast cancer and arthritis, respectively.

【 授权许可】

CC BY   
© Shanghai Jiao Tong University 2023

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