Tools for probing host-bacteria interactions in the gut microenvironment: From molecular to cellular levels

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:30

Review

Wodzanowski, Kimberly A.¹  Cassel, Samantha E.²  Grimes, Catherine L.^1,3  Kloxin, April M.^2,4 

[1] Univ Delaware, Chem & Biochem, Newark, DE 19716 USA

[2] Univ Delaware, Chem & Biomol Engn, Newark, DE 19716 USA

[3] Univ Delaware, Biol Sci, Newark, DE 19716 USA

[4] Univ Delaware, Mat Sci & Engn, Newark, DE 19716 USA

关键词: Biomaterials;    Co-culture;    Gastrointestinal;    Bacterial peptidoglycan;    Bioorthogonal chemistry;    Synthetic culture models;    Host-bacteria interactions;    Cell-matrix interactions;   

DOI  :  10.1016/j.bmcl.2020.127116

来源: Elsevier

【 摘 要 】

Healthy function of the gut microenvironment is dependent on complex interactions between the bacteria of the microbiome, epithelial and immune (host) cells, and the surrounding tissue. Misregulation of these interactions is implicated in disease. A range of tools have been developed to study these interactions, from mechanistic studies to therapeutic evaluation. In this Digest, we highlight select tools at the cellular and molecular level for probing specific cell-microenvironment interactions. Approaches are overviewed for controlling and probing cell-cell interactions, from transwell and microfluidic devices to engineered bacterial peptidoglycan fragments, and cell-matrix interactions, from three-dimensional scaffolds to chemical handles for in situ modifications.

【 授权许可】

Free